英花枇杷颗粒中黄芩苷含量测定的研究  

Study on the Determination of Baicalin in Yinghua Loquat Granules

作　　者：胡丽 Hu Li(Taihe County Market Supervision and Inspection Institute,Fuyang 236600,China)

机构地区：[1]太和县市场监督检验所,安徽阜阳236600

出　　处：《广东化工》2024年第14期162-164,共3页Guangdong Chemical Industry

摘　　要：目的:建立HPLC法测定英花枇杷颗粒中黄芩苷的含量,为制剂的质量控制提供方法。方法:运用HPLC法测定制剂中黄芩苷的含量,C_(18)柱,流动相甲醇-0.1%磷酸溶液(47∶53),波长280 nm。结果:制剂中黄芩苷的分离度较好,进样量(0.04635μg~0.3708μg)与峰面积呈较好的线性关系,Y=62.727X-0.0286(R^(2)=1);平均回收率96.95%,RSD=0.41%(n=6)。结论:方法专属性强,可作为本品中黄芩苷的含量测定方法用于质量控制。Objective:to establish a HPLC method for the determination of Baicalin in Yinghua pipa granules and to provide a method for the quality control of the preparation.Methods:the content of Baicalin in the preparation was determined by HPLC,C_(18) column,and mobile phase methanol-0.1%phosphoric acid solution(47∶53),wavelength 280 nm.Results:the separation degree of Baicalin in the preparation was good,and there was a good linear relationship between the injection volume(0.04635μg~0.3708μg)and the peak area,Y=62.727x-0.0286(r^(2)=1),the average recovery was 96.95%,RSD=0.41%(N=6).Conclusion:the method is specific and can be used for the quality control of Baicalin.

关 键 词：英花枇杷颗粒 黄芩苷 含量测定 HPLC 

分 类 号：TQ[化学工程]