大叶山楝中二萜类化学成分及其抗炎活性研究  

Diterpenoids from Aphanamixis polystachya and their anti-inflammatory activities

作　　者：陈彦伍 秦天丽 吴石丽 蒋华夷 何红平 CHEN Yanwu;QIN Tianli;WU Shili;JIANG Huayi;HE Hongping(College of Traditional Chinese Medicine,Yunnan University of Chinese Medicine,Kunming 650500,China)

机构地区：[1]云南中医药大学中药学院,云南昆明650500

出　　处：《中草药》2024年第14期4656-4662,共7页Chinese Traditional and Herbal Drugs

基　　金：云南省科技重大专项(202002AA100007)。

摘　　要：目的研究大叶山楝Aphanamixis polystachya枝叶的化学成分及其抗炎活性。方法采用硅胶、C18反相硅胶、Sephadex LH-20凝胶及MCI等多种柱色谱技术进行分离纯化,根据理化性质结合波谱数据鉴定化合物结构。采用脂多糖(lipopolysaccharide,LPS)诱导的RAW 264.7巨噬细胞炎症模型,对化合物进行抗炎活性评价。结果从大叶山楝枝叶中分离得到11个二萜类化合物,分别鉴定为(4S)-4-[(E)-11-(15,15-dimethyl-14-oxo-14,15-dihydrofuran-12-yl)-11,18-dihydroxy-7-methyloct-6-en-5-yl]-3-methyl-1(4H)-furanone(1)、nemoralisin B(2)、aphapolin A(3)、nemoralisin G(4)、nemoralisin J(5)、nemoralisin I(6)、nemoralisin H(7)、nemoralisin D(8)、nemoralisin A(9)、aphanamene B(10)和反式植醇(11)。化合物11对LPS诱导的RAW 264.7细胞内NO的生成有微弱抑制作用,其半数抑制浓度(median inhibition concentration,IC50)值为428.7μmol/L。结论化合物1为新化合物,命名为陵水暗罗素O(nemoralisin O);化合物3、8和11为首次从大叶山楝中分离得到;化合物11显示出微弱的抗炎活性。Objective To study the chemical constituents from the stems and leaves of Aphanamixis polystachya and their antiinflammatory activities.Methods The compounds were isolated and purified by various column chromatography techniques,such as silica gel,C18 reversed-phase silica gel,Sephadex LH-20 and MIC,and their structures were identified by physiochemical properties and spectroscopic data.The LPS-induced RAW 264.7 macrophage inflammation model was used to evaluate the anti-inflammatory activity.Results Eleven diterpenoids were isolated,which were elucidated as nemoralisin O(1),nemoralisin B(2),aphapolin A(3),nemoralisin G(4),nemoralisin J(5),nemoralisin I(6),nemoralisin H(7),nemoralisin D(8),nemoralisin A(9),aphanamene B(10),and trans-phytol(11).Compound 11 exhibited weak inhibitory activity against LPS-induced NO production with a half-maximal inhibitory concentration(IC50)value of 428.7μmol/L.Conclusion Compound 1 is identified as a new compound and named nemoralisin O(1).Compounds 3,8,and 11 were isolated from this plant for the first time.Compound 11 showed weak antiinflammatory activity.

关 键 词：大叶山楝 二萜 抗炎活性 陵水暗罗素O 反式植醇 aphapolin A nemoralisin D 

分 类 号：R284.1[医药卫生—中药学]