草珊瑚叶片转录组特征分析及活性成分合成相关基因挖掘  

Transcriptome characteristic and discovery of related genes involved in active ingredients biosynthesis of Sarcandra glabra leaves

作　　者：蓝祖栽[1] 胡营[1] 闫志刚[1] 李刚[1] 韦筱媚[1] 韦桂丽 马梅冰 唐美琼[1] LAN Zuzai;HU Ying;YAN Zhigang;LI gang;WEI Xiaomei;WEI Guili;MA Meibing;TANG Meiqiong(Guangxi Engineering Research Center of TCM Resource Intelligent Creation,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement,National Engineering Research Center for Southwest Endangered Medicinal Materials Resources Development,National Center for TCM Inheritance and Innovation,Guangxi Botanical Garden of Medicinal Plants,Nanning 530023,China)

机构地区：[1]广西壮族自治区药用植物园、国家中医药传承创新中心、西南濒危药材资源开发国家工程研究中心、广西药用资源保护与遗传改良重点实验室/广西壮族自治区中药资源智慧创制工程研究中心,广西南宁530023

出　　处：《中草药》2024年第14期4860-4870,共11页Chinese Traditional and Herbal Drugs

基　　金：广西科技重大专项(桂科AA22096021);广西创新驱动发展专项(桂科AA18242040);广西药用植物保育人才小高地自主研究项目(GXYYXGD202201)。

摘　　要：目的探索草珊瑚Sarcandra glabra叶片活性成分生物合成分子基础,合理的开发利用草珊瑚资源。方法采用BGISEQ-500测序平台测定草珊瑚叶片的转录组,对经过滤和Trinity组装得到的unigene进行生物信息学分析。结果共获得了75862个unigenes,平均长度980 bp,其中42369个unigenes在7大公共数据库中得到成功注释,占总基因数的55.85%。21801个unigenes被注释于细胞组分、分子功能和生物学过程3大类共55个GO条目京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)富集到2条可能与药效物质形成密切相关的通路,分别是萜类和聚酮化合物的代谢和其他次生代谢物生物合成。通过进一步挖掘发现,其中47个unigenes可能参与萜类化合物骨架生物合成,14个unigenes可能参与倍半萜类化合物生物合成,62个unigenes可能参与黄酮类化合物生物合成。另外,转录组序列中共检测到19690个SSR位点,其中二核苷酸重复类型所占比例最高,达到了46.63%;针对所有的SSR位点,共设计出11209对引物。结论很好地扩展了草珊瑚的转录组信息,为进一步解析草珊瑚活性成分生物合成途径提供了基因资源,促进草珊瑚分子育种的研究。Objective To explore the molecular basis of active ingredients biosynthesis in the leaves of Sarcandra glabra,and exploit this resource reasonably.Methods S.glabra leaf was analyzed by transcriptome sequencing with the BGISEQ-500 platform.After filtration and the Trinity assembly,the unigenes were analyzed by bioinformatics.Results A total of 75862 unigenes with an average length of 980 bp were obtained.Among them,42369 unigenes,accounting for 55.85%of the total,were successfully annotated in seven public databases.A total of 21801 unigenes were categorized into 55 GO terms among three categories:biological process,cellular component and molecular function.KEGG enrichment analyses shown that there were two pathways,namely“metabolism of terpenoids and polyketides”and“biosynthesis of other secondary metabolites”,may be closely related to the formation of pharmacodynamic basis.Moreover,it was discovered that 47 unigenes,14 unigenes and 62 unigenes might participate in terpenoid backbone,sesquiterpenoids and flavonoid biosynthesis,respectively.In addition,a total of 19690 SSR sites were detected in the transcriptome sequences,of which the highest proportion of di-nucleotides was 46.63%.A total of 11209 primer pairs were designed for all SSR sites.Conclusion This work extend the transcriptome information of S.glabra,which will provide gene resources for further study the biosynthesis pathway of active ingredients in S.glabra and facilitate molecular breeding in this species.

关 键 词：草珊瑚 转录组 次生代谢 生物合成 SSR 

分 类 号：R286.12[医药卫生—中药学]