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作 者:李天杰 郑伟[1] 苗圃[2] 王惠[2] 汪孝国[2] 周俊学[2] 康业斌[1] 徐建强[1] LI Tianjie;ZHENG Wei;MIAO Pu;WANG Hui;WANG Xiaoguo;ZHOU Junxue;KANG Yebin;XU Jianqiang(College of Horticulture and Plant Protection,Henan University of Science and Technology,Luoyang 471000,Henan Province,China;Luoyang Branch of Henan Provincial Tobacco Company,Luoyang 471000,Henan Province,China)
机构地区:[1]河南科技大学园艺与植物保护学院,河南洛阳471000 [2]河南省烟草公司洛阳市公司,河南洛阳471000
出 处:《农药学学报》2024年第4期825-830,共6页Chinese Journal of Pesticide Science
基 金:河南省烟草公司洛阳市公司科技项目(LYKJ202109).
摘 要:为评估四氟醚唑在烟草白粉病上的应用潜力,采用孢子萌发抑制法测定了四氟醚唑对烟草高氏白粉菌的室内毒力,并观察了其对烟草高氏白粉菌形态的影响;通过盆栽试验测定了四氟醚唑对烟草白粉病的温室防治效果。结果表明:四氟醚唑对3株烟草高氏白粉菌菌株分生孢子萌发有强烈的抑制作用,其EC50值分别为0.3422、0.3561和0.4105μg/mL;对芽管伸长的抑制作用明显,其EC50值分别为1.7466、1.3988和1.3229μg/mL。光学显微镜观察结果表明:四氟醚唑对烟草高氏白粉菌分生孢子无致畸作用,但能使其初生菌丝畸形。温室防效试验结果表明:4%四氟醚唑水乳剂在有效成分1.61、3.22和5.36 g/L剂量下,喷施处理烟苗14 d后,保护作用的防效在76.62%~95.67%之间,治疗作用的防效在71.42%~90.91%之间。In order to evaluate the application potential of tetraconazole in the prevention and control of tobacco powdery mildew,the spore germination method was used to test the toxicity of tetraconazole on spore germination of Golovinomyces tabaci,and its effect on the microscopic morphology of G.tabaci.The pot experiments were conducted to test the control effect of tetraconazole on tobacco powdery mildew in the greenhouse.The results indicated that tetraconazole had a strong inhibitory effect on conidial germination of three G.tabaci strains,and the EC50 values were 0.3422,0.3561 and 0.4105μg/mL,respectively.The EC50 values for inhibition of germ tube elongation were 1.7466,1.3988,and 1.3229μg/mL,respectively.Microscopic observation results indicated that tetraconazole had no teratogenic effect on conidia,but it could make the primary hyphae thickened and bifurcated.The results of the greenhouse control test showed that after 14 days of treating tobacco seedlings with 4%tetraconazole water emulsion at doses of 1.61,3.22,and 5.36 g/L,respectively,the protective effect ranged from 76.62%to 95.67%,and the therapeutic effect ranged from 71.42%to 90.91%.
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