制刺猬皮免疫调节白蛋白的多肽鉴定与分析  

Identification and Analysis of Polypeptides of Immunomodulatory Albumin from Zhiciweipi

作　　者：韩君 Han Jun(Beijing Tcmages Pharmaceutical Co.,Ltd.,Beijing 101301,China)

机构地区：[1]北京康仁堂药业有限公司,北京101301

出　　处：《大理大学学报》2024年第8期57-62,共6页Journal of Dali University

摘　　要：目的:对制刺猬皮免疫调节白蛋白(ALB)的多肽进行鉴定与分析。方法:应用LC-MS/MS、生物信息学软件结合同源序列比对和进化分析对制刺猬皮酶解后的成分鉴定,并对筛选得到免疫调节核心蛋白及多肽进行分析。结果:ALB是免疫调节关键蛋白,其中具有活性的多肽蛋白序列为RHPYFYAPELLYF,进化树分析显示欧洲刺猬与埃氏象鼩的ALB蛋白100%同源,亲缘关系最近,同源序列比对分析显示两物种的ALB蛋白都含608个氨基酸,氨基酸序列极为相似,具有活性的多肽蛋白序列RHPYFYAPELLYF无差异。结论:ALB的多肽在欧洲刺猬和埃氏象鼩中生物学功能相似,这为珍稀动物的临床替代性提供了部分基础证据。Objective:To identify and analyze the polypeptides of immunomodulatory albumin(ALB)from the Zhiciweipi.Methods:LC-MS/MS and bioinformatics software combined with homologous sequence comparison and evolutionary analysis were applied to identifying the components after enzymolysis of Zhiciweipi and analyzing the immunomodulatory core proteins and peptides obtained from the screening.Results:ALB was the key immunomodulatory protein and the active peptide protein sequence was RHPYFYAPELLYF.Evolutionary tree analysis indicated that the ALB of Erinaceus europaeus was 100%homologous with that of Elephantulus edwardii,with the closest genetic relationship.The homologous sequence comparison analysis showed that ALB of both species contained 608 amino acids,with very similar amino acid sequence,and the active polypeptide protein sequence RHPYFYAPELLYF had no difference.Conclusion:Polypeptides of ALB in Erinaceus europaeus and Elephantulus edwardii have similar biological functions,and this provides part of the basic evidence for the clinical substitutability of rare animals.

关 键 词：制刺猬皮 生物信息学 免疫活性 进化分析 珍稀动物药材 替代品 

分 类 号：R931.74[医药卫生—生药学]