生殖道解脲支原体感染及男性年龄对精子DNA碎片指数、精液常规参数的影响  

The impact of ureaplasma urealyticum infection of reproductive tract and age on sperm DNA fragmentation index and routine semen parameters

在线阅读下载全文

作  者:袁康凯[1] 王奕君 江凡[1] 池丽滢 庾伟坚 陈献 YUAN Kang-kai;WANG Yi-jun;JIANG Fan;CHI Li-ying;YU Wei-jian;CHEN Xian(Laboratory of Medicine,Shenzhen Baoan Women’s and Children’s Hospital,Shenzhen 518102)

机构地区:[1]深圳市宝安区妇幼保健院检验科,深圳518102

出  处:《生殖医学杂志》2024年第8期1058-1063,共6页Journal of Reproductive Medicine

基  金:广东省深圳市宝安区科技创新计划基础研究项目(2022JD111,2020JD462)。

摘  要:目的探讨生殖道解脲支原体(Uu)感染及男性年龄对精子DNA碎片指数(DFI)及精液常规参数的影响。方法收集2019年1月至2023年7月于深圳市宝安区妇幼保健院体检科和生殖健康科进行婚前或孕前保健检查的男性7106例为研究对象,所有研究对象进行精液常规参数检查,并采用培养法和流式细胞术分别进行精液Uu检测与精子DFI分析。依据年龄截点将研究对象分成3组(<30岁、30~35岁、>35岁),分析Uu检出情况及Uu阳性男性中不同年龄组间精液常规参数和精子DFI差异。结果近5年男性生殖道Uu总体检出率为11.89%(845/7106),除2020年检出率略有下降外,整体呈逐年上升趋势;生殖道Uu检出率无明显的年龄和季节性差异。Uu阳性男性不同年龄组间比较发现,相比<30岁和30~35岁组,>35岁组的精子存活率、前向运动精子百分率、精子总数显著降低(P<0.05),精子DFI显著增高(P<0.05)。Uu阳性男性年龄与精子DFI呈正相关关系(r=0.26,P=0.0002)。Logistic回归分析结果显示,年龄[OR=1.393,95%CI(1.002,1.935),P=0.040]、精子存活率[OR=0.746,95%CI(0.608,0.916),P=0.005]和精子总数[OR=0.984,95%CI(0.968,0.999),P=0.040]是Uu阳性男性精子DFI的独立影响因素(P<0.05)。结论生殖道Uu阳性的高龄男性精液常规参数欠佳,且精子DFI显著增加。Uu检测可作为孕前男性精液质量筛查的候选指标,为全面客观评价男性生育力提供更多实验室证据。Objective:To explore the effects of ureaplasma urealyticum(Uu)infection of reproductive tract and age on sperm DNA fragmentation index(DFI)and routine semen parameters.Methods:A total of 7106 men who underwent premarital medical examination and preconception care examination on Physical Examination Department and Reproductive Health Department of Shenzhen Baoan Women’s and Children’s Hospital from January 2019 to July 2023 were recruited.Human semen was collected to analyze conventional sperm parameters and also Uu infection and sperm DFI with the cultivation method and flow cytometry.All the cases were classified into three groups based on their age for less than 30y,30-35y and over 35y.The discrepancy with various sperm parameters was evaluated.Results:Uu-positive rate was 11.89%among 5 years with uptrend overall while dropping at 2020.There were no significant differences in a positive rate of Uu among different seasons and ages.As for Uu-positive men,compared with those aged less than 30y and 30-35y,men aged over 35y had significantly lower sperm survival rate,percentage of forward sperm motility and total sperm count(P<0.05)as well as significantly higher sperm DFI(P<0.05).The age was positively correlated with sperm DFI among Uu-positive men(r=0.26,P=0.0002).Logistic regression results showed that age[OR=1.393,95%CI(1.002,1.935),P=0.040],sperm survival rate[OR=0.746,95%CI(0.608,0.916),P=0.005]and total sperm count[OR=0.984,95%CI(0.968,0.999),P=0.040]were independently risk factors for sperm DFI among Uu-positive men(P<0.05).Conclusions:Uu positive,along with advanced ages,notably resulted in abnormal routine semen characteristics and higher sperm DFI.Uu testing could be considered as a candidate screening indicator for semen quality as well as laboratory evidence for men’s fertility evaluation.

关 键 词:解脲支原体 年龄 精子DNA碎片指数 精液常规参数 

分 类 号:R446.19[医药卫生—诊断学]

 

参考文献:

正在载入数据...

 

二级参考文献:

正在载入数据...

 

耦合文献:

正在载入数据...

 

引证文献:

正在载入数据...

 

二级引证文献:

正在载入数据...

 

同被引文献:

正在载入数据...

 

相关期刊文献:

正在载入数据...

相关的主题
相关的作者对象
相关的机构对象