超声波法提取恒山黄芪叶茶黄酮最佳工艺研究  

Study on the Optimal Extraction Process of Flavonoids from Hengshan Radix Astragali Leaf Tea by Ultrasonic Method

作　　者：张永芳[1,2] 杨文华 张东旭[1,2] 李侠[1,2] 郭浩然 王碧玉 ZHANG Yongfang;YANG Wenhua;ZHANG Dongxu;LI Xia;GUO Haoran;WANG Biyu(School of Agronomy and Life Sciences,Shanxi Datong University,Datong Shanxi,037009;Facility Agriculture Technology Research and Development Center,Shanxi Datong University,Datong Shanxi,037009;Shanxi Hengshan Tea Co.,Ltd.,Datong Shanxi,037009)

机构地区：[1]山西大同大学农学与生命科学学院,山西大同037009 [2]山西大同大学设施农业技术研发中心,山西大同037009 [3]山西恒山茶叶有限公司,山西大同037009

出　　处：《山西大同大学学报（自然科学版）》2024年第4期85-90,共6页Journal of Shanxi Datong University(Natural Science Edition)

基　　金：山西省高等学校科技创新项目[2022L459];大同市科技攻关项目[2020044];山西大同大学博士科研启动经费[2021-B-08]。

摘　　要：目的探索超声波法提取黄芪叶茶中黄酮的最优方法。方法利用超声波辅助提取法结合单因素试验和正交试验,确定提取恒山黄芪叶茶中黄酮的最佳提取工艺。结果因素影响力排序:料液比>乙醇浓度>超声波功率>提取温度>提取时间。得到最佳提取工艺为:料液比为1∶35 g/mL,乙醇浓度为60%,超声波功率为280 W,提取温度为60℃,提取时间为45 min。结论在此最佳条件下,恒山黄芪叶茶中总黄酮的提取率为14.302%。Objective To explore the optimal method for ultrasonic extraction of flavonoids from Radix Astragali leaf tea.Methods Using ultrasound assisted extraction method combined with single factor experiment and orthogonal experiment,the optimal extraction process for flavonoids in Hengshan Radix Astragali leaf tea was determined.Result The factors were ranked as follows:material-liquid ratio>ethanol concentration>ultrasonic power>extraction temperature>extraction time;The optimal extraction process was as follows:the material-liquid ratio was 1∶35 g/mL,the ethanol concentration was 60%,the ultrasonic power was 280 W,the extraction temperature was 60°C,and the extraction time was 45 min.Conclusion Under these optimal conditions,the extraction rate of total flavonoids in Hengshan Radix Astragali leaf tea was 14.302%.

关 键 词：超声波辅助提取 恒山黄芪 叶茶 黄酮 

分 类 号：Q58[生物学—生物化学]