甜瓜品系萌发期耐盐性鉴定及筛选  

Identification and selection of the salt tolerance of melon varieties at germination stage

作　　者：刘鹏 杨文强 王崇启[1] 焦自高[1] 高超[1] 孙建磊[1] 董玉梅[1] LIU Peng;YANG Wenqiang;WANG Chongqi;JIAO Zigao;GAO Chao;SUN Jianlei;DONG Yumei(Institute of Vegetables,Shandong Academy of Agricultural Sciences,Jinan 250100,Shandong,China)

机构地区：[1]山东省农业科学院蔬菜研究所,济南250100

出　　处：《中国瓜菜》2024年第8期34-50,共17页China Cucurbits And Vegetables

基　　金：山东省自然科学基金面上项目(ZR2021MC101);国家现代农业产业技术体系建设专项资金项目(CARS-25);山东省农业良种工程项目(2022LZGCQY006);国家盐碱地中心“揭榜挂帅”项目(GYJ2023004)。

摘　　要：为了解不同甜瓜品系的耐盐程度,明确萌发期耐盐性评价指标,采用0、50、100、150、200、250 mmol·L^(-1)6个浓度梯度的NaCl溶液对8份甜瓜材料进行5 d萌发期盐胁迫处理,筛选出200 mmol·L^(-1)为盐胁迫适宜浓度;用0和200 mmol·L^(-1)NaCl溶液对213份甜瓜材料进行5 d萌发期胁迫处理,调查各甜瓜材料的发芽势、发芽率、发芽指数、根长、鲜质量、活力指数等6个指标,利用相关性分析、主成分分析、隶属函数分析、系统聚类等方法对其进行耐盐性评价。研究结果表明,主成分分析法将6个耐盐指标综合为1个主成分,可代表甜瓜耐盐性79.23%的原始数据信息量;发芽率、发芽势、发芽指数、根长为甜瓜萌发期耐盐性材料鉴定的重要指标;可将207份材料分为四个等级,分别为耐盐、较耐盐、盐敏感、盐极敏感材料各39、73、40、55份。研究结果为耐盐甜瓜材料鉴定、培育和耐盐基因挖掘提供材料和方法支撑。In order to understand the salt tolerance of different melon varieties and clarify the evaluation indicators of salt tolerance during germination,8 melon varieties were subjected to 5 days of salt stress treatment during germination using NaCl solutions with 6 concentration gradients of 0,50,100,150,200,and 250 mmol·L^(-1),and 200 mmol·L^(-1) was selected as the appropriate concentration for salt stress.213 varieties were subjected to 5 days germination stress treatment with 0 and 200 mmol·L^(-1) NaCl solutions and 6 indicators,including germination potential,germination rate,germination index,root length,fresh mass,and vitality index,were investigated for each melon variety.Correlation analysis,principal component analysis,membership function analysis,and systematic clustering were used to evaluate their salt tolerance.The research results indicate that the principal component analysis method integrates 6 salt tolerance indicators into 1 principal component,which can represent 79.23%of the original data information of salt tolerance.Germination rate,germination potential,germination index,and root length are important indicators for identifying salt tolerant varieties during the germination period.207 materials can be divided into 4 levels successfully,namely 39 salt tolerant,73 relatively salt tolerant,40 salt sensitive,and 55 salt extremely sensitive varieties.This study provides material and methodological support for the identification,cultivation,and gene mining of salt tolerant melon varieties.

关 键 词：甜瓜 NACL胁迫 品种鉴定 萌发期 

分 类 号：S652[农业科学—果树学]