从肝论治取穴针灸通过调控JAK2/STAT3途径对膝骨关节炎大鼠关节软骨的保护作用  

Protective Effect of Acupuncture and Moxibustion on Articular Cartilage in Rats with Knee Osteoarthritis through Regulation of JAK2/STAT3 Pathway Based on Liver Theory and Treatment

作　　者：樊远志[1] 张峻峰[1] 陈威[1] 李艳[1] 吴耀持[1] FAN Yuanzhi;ZHANG Junfeng;CHEN Wei;LI Yan;WU Yaochi(Shanghai Sixth People's Hospital,Shanghai 200233,China)

机构地区：[1]上海市第六人民医院,上海200233

出　　处：《中医药导报》2024年第7期45-48,53,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：上海市名老中医学术经验研究工作室建设项目(SHGZS-202219);上海市中医药管理局治未病技术处方与规范项目[ZY(2021-2023)-0104-02-JS-12];上海市进一步加快中医药传承创新发展三年行动计划-上海市中西医结合旗舰医院项目[ZY(2021-2023)-0205-04]。

摘　　要：目的:探究从肝论治取穴针灸通过调控JAK2/STAT3途径对膝骨关节炎(OA)大鼠关节软骨的保护作用。方法:60只SD大鼠随机分为对照组、OA组、OA+针灸组,每组20只,通过手术构建右膝OA模型。OA+针灸组大鼠接受从肝论治取穴针灸4周。检测软骨退化情况、关节炎症,以及JAK2/STAT3转录和蛋白水平。结果:OA组大鼠OARSI评分高于对照组(P<0.05);干预后,OA+针灸组大鼠OARSI评分低于OA组(P<0.05)。OA组大鼠透明软骨(HC)厚度低于对照组,钙化软骨(CC)厚度高于对照组(P<0.05);干预后,OA+针灸组大鼠HC厚度高于OA组,CC厚度低于OA组(P<0.05)。OA组大鼠白细胞介素-1β(IL-1β)、IL-6、JAK2 mRNA、STAT3 mRNA、JAK2蛋白、STAT3蛋白水平均高于对照组(P<0.05);干预后,OA+针灸组大鼠IL-1β、IL-6、JAK2 mRNA、STAT3 mRNA、JAK2蛋白、STAT3蛋白水平均低于OA组(P<0.05)。结论:从肝论治取穴针灸可通过调控JAK2/STAT3途径发挥对OA大鼠膝关节软骨的保护作用。Objective:To explore the protective effect of acupuncture and moxibustion on the articular cartilage of rats with knee osteoarthritis(OA)by regulating the JAK2/STAT3 pathway based on liver theory and treatment.Methods:Totally 60 SD rats were randomely divided into control group,OA group,OA+acupuncture group,20 rats in each group.The right knee OA model was surgically constructed.The rats in the OA+acupuncture group received acupuncture at acupoints selected from the liver theory for 4 weeks.Cartilage degeneration,joint inflammation,and JAK2/STAT3 transcription and protein levels were detected.Results:OA group showed higher OARSI score than control group(P<0.05).OA+acupuncture group showed lower OARSI score than OA group(P<0.05).OA group showed lower thickness of hyaline cartilage(HC)than control group,while higher thickness of calcified cartilage(CC)than control group(P<0.05).OA+acupuncture group showed higher thickness of HC than OA group,while lower thickness of CC than OA group(P<0.05).OA group showed higher IL-1β,IL-6,JAK2 mRNA,STAT3 mRNA,JAK2 protein and STAT3 protein than control group(P<0.05).OA+acupuncture group showed lower IL-1β,IL-6,JAK2 mRNA,STAT3 mRNA,JAK2 protein and STAT3 protein than OA group(P<0.05).Conclusion:Acupuncture and moxibustion based on acupoints selected from liver theory play a protective role on knee articular cartilage in knee OA rats by regulating JAK2/STAT3 pathway.

关 键 词：膝骨关节炎 从肝论治 针灸 JAK2 STAT3 软骨 大鼠 

分 类 号：R285.5[医药卫生—中药学]