四川不同产地烟叶中黄酮类物质的代谢特征比较  

Comparative study on metabolic characteristics of flavonoids in tobacco leaves from different growing areas in Sichuan Province

作　　者：李晓阳 陈爱国[2] 庞夙 张海燕 靳冬梅 邱军[2] 魏玉西[1] 曹建敏[2] LI Xiaoyang;CHEN Aiguo;PANG Su;ZHANG Haiyan;JIN Dongmei;QIU Jun;WEI Yuxi;CAO Jianmin(Qingdao University,Qingdao 266071,Shandong,China;Tobacco Research Institute of CAAS,Qingdao 266101,Shandong,China;Sichuan Provincial Tobacco Company of CNTC,Chengdu 610017,China)

机构地区：[1]青岛大学,山东省青岛市266071 [2]中国农业科学院烟草研究所,山东省青岛市266101 [3]中国烟草总公司四川省公司,成都市高新区610017

出　　处：《烟草科技》2024年第6期33-42,共10页Tobacco Science & Technology

基　　金：中国烟草总公司四川省公司项目“优质烟叶特征物质解析及代谢通路研究”(SCYC202118);中国农业科学院科技创新工程(ASTIP-TRIC07)。

摘　　要：为明确四川四大烟叶产地的烟叶在黄酮类代谢物及代谢途径方面的差异,采用LC-MS对四川四大烟叶产地成熟期烟叶中的黄酮类代谢产物进行代谢组学研究,结合主成分分析(PCA)和正交偏最小二乘判别分析(OPLS-DA)等多元统计分析和代谢途径分析,探讨产地对烟草代谢产物的影响。结果显示:①共检测出黄酮类代谢物290种,分为黄酮醇、黄酮、二氢黄酮、花青素、二氢黄酮醇、查耳酮、异黄酮、黄烷醇类和二氢异黄酮等9类。②筛选出凉山与攀枝花烟叶之间的差异代谢物18种,凉山与宜宾烟叶之间的差异代谢物30种,凉山与广元烟叶之间的差异代谢物38种,攀枝花与宜宾烟叶之间的差异代谢物19种,攀枝花与广元烟叶之间的差异代谢物45种,宜宾与广元烟叶之间的差异代谢物23种。③OPLS-DA结果表明,不同产地烟叶区分明显,均有显著样本聚集区,说明产地对烟叶黄酮类代谢的影响很大。④代谢通路结果表明,四川不同产地的烟叶差异代谢物主要注释与富集在代谢途径、黄酮和黄酮醇生物合成通路、次生代谢物生物合成通路、异黄酮生物合成通路、类黄酮生物合成通路、花青素生物合成通路等6大代谢通路中。烟叶产地不同,黄酮类代谢产物也各不相同,同时也赋予了烟叶不同的香韵。To clarify the differences in flavonoid metabolites and metabolic pathways of tobacco leaves from four major tobacco growing areas in Sichuan Province,the metabolomics of flavonoid metabolites in mature tobacco leaves from these regions were studied by LC-MS.The different effects on tobacco metabolites were investigated by combining principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)with metabolic pathway analysis.The results showed that:1)A total of 290 flavonoid metabolites were detected,which were divided into 9 categories,including flavonols,flavonoids,dihydroflavonoids,anthocyanins,dihydroflavonols,chalcones,isoflavones,flavanols,and dihydroisoflavones.2)There were 18 different metabolites between Liangshan and Panzhihua leaves,30 between Liangshan and Yibin leaves,38 between Liangshan and Guangyuan leaves,19 between Panzhihua and Yibin leaves,45 between Panzhihua and Guangyuan leaves,23 between Yibin and Guangyuan leaves.3)The OPLS-DA results showed that tobacco leaves from the different areas could be clearly distinguished and there were significant sample accumulation by areas,indicating that the growing area greatly influenced the flavonoid metabolism in tobacco leaves.4)The metabolic pathway analysis showed that the differential metabolites of tobacco leaves from these different areas in Sichuan were mainly annotated and enriched in six major metabolic pathways,including metabolic pathways,flavonoid and flavonol biosynthesis pathways,secondary metabolite biosynthesis pathways,isoflavone biosynthesis pathways,flavonoid biosynthesis pathways,and anthocyanin biosynthesis pathways.The flavonoid metabolites in tobacco leaves from the different growing areas were different,giving these tobacco leaves different flavor notes.

关 键 词：四川烟叶 黄酮类代谢物 代谢组学 代谢通路 

分 类 号：TS413[农业科学—烟草工业]