一株新分离黑曲霉对糖蜜酵母废水的脱色研究  

Decolorization of molasses yeast wastewater by a newly isolated Aspergillus niger

作　　者：程宁 夏楠 卫燕 沈欣悦 李采坪 陈勇 CHENG Ning;XIA Nan;WEI Yan;SHEN Xinyue;LI Caiping;CHEN Yong(Department of Food and Chemical Engineering,Liuzhou Institute of Technology,Liuzhou 545616,China;School of Environment and Food Engineering,Liuzhou Vocational and Technical College,Liuzhou 545000,China;Special Industrial Wastewater Biological Treatment Technology Experimental Center,Liuzhou Vocational&Technical College,Liuzhou 545000,China)

机构地区：[1]柳州工学院食品与化学工程学院,广西柳州545616 [2]柳州职业技术学院环境与食品工程学院,广西柳州545000 [3]柳州职业技术学院特色产业废水生物处理技术实验中心,广西柳州545000

出　　处：《化工环保》2024年第4期523-530,共8页Environmental Protection of Chemical Industry

基　　金：广西高校中青年教师科研基础能力提升项目(“广西特色产业废水脱色技术机制研究”2022KY1035,“工业废弃树脂功能化再生及其应用于染料废水处理研究”2024KY1801);柳州市科技计划项目(2022CCC0202,2024SB0505G004);国家级(广西)大学生创新创业训练计划项目(202213639001)。

摘　　要：从受糖蜜酵母废水污染的土壤中分离出2株具有脱色活性的真菌菌株,通过菌株形态分析和基因型鉴定,确定其中具有相对较大脱色潜力的菌株为黑曲霉(Aspergillus niger),将其命名为TMZ1。以碳源、氮源、初始pH为关键因素,考察了TMZ1对实际生产过程产生的糖蜜酵母废水的脱色效果,并分析了脱色机理。实验结果表明,将TMZ1接种于由18%糖蜜酵母废水、3.0%蔗糖、0.2%NaNO_(3)、0.1%KH_(2)PO_(4)、0.05%MgSO_(4)·7H_(2)O等组成的培养基中,OD值为3,初始pH为5.5,于39℃培养4 d后,脱色率可达52.9%,COD去除率为80.1%,细胞干重高达14.100 g/L。凝胶过滤色谱法分析显示,在脱色过程中大分子量和小分子量组分均得到有效去除。该菌株的脱色机理由生物吸附和生物降解共同组成,且生物降解发挥主要作用。Two fungus strains with decolorization activity were isolated from soil samples contaminated with molasses wastewater.After morphology analysis and genotype identification,the strain with relatively high decolorization potential was determined as Aspergillus niger and named TMZ1.This study analyzed the decolorization effect and mechanism of this strain on molasses yeast wastewater in actual production by key factors such as carbon source,nitrogen source,and initial pH value.The results show that after TMZI was inoculated into the culture medium composed of 18%molasses yeast wastewater,3%sucrose,0.2%NaNO_(3),0.1%KH_(2)PO_(4),0.05%MgSO_(4)·7H_(2)O and others,with OD value of 3 and initial pH of 5.5,cultured at 39℃for 4 d,the TMZI decolorization rate reaches 52.9%,COD is reduced by 80.1%and cell dry weight is up to 14.100 g/L.Gel filtration chromatography shows that both high molecular weight and low molecular weight components are effectively removed in the decolorization process.The decolorization mechanism of this strain is composed of biological adsorption and biodegradation,with biodegradation playing a major role.

关 键 词：黑曲霉 脱色 糖蜜酵母废水 

分 类 号：X792[环境科学与工程—环境工程]