油橄榄幼嫩茎段组培快繁技术研究  

Study on tissue culture and rapid propagation technology of tender stem segments of Olea europaea

作　　者：姜丽琼[1] 浣杰 徐志萍[1] 何周建 李文俊[1] JIANG Liqiong;HUAN Jie;XU Zhiping;HE Zhoujian;LI Wenjun(Chengdu Academy of Agricultural and Forestry Sciences,Chengdu 611130,China;Bazhong Enyang District Agricultural and Rural Bureau,Bazhong 636064,China;College of Life Sciences,Sichuan University,Chengdu 611130,China)

机构地区：[1]成都市农林科学院,成都611130 [2]巴中市恩阳区农业农村局,四川巴中636064 [3]四川大学生命科学学院,成都611130

出　　处：《四川林业科技》2024年第4期20-25,共6页Journal of Sichuan Forestry Science and Technology

摘　　要：以阿贝基纳优良单株幼嫩茎段为试验材料,通过对油橄榄外植体灭菌、基本培养基、生长调节剂等因素对油橄榄组织培养技术影响的研究,试验结果表明:使用75%乙醇30 s、0.1%HgCl_(2) 10 min进行外植体消毒处理时获得率最高,达36.15%;改良MS培养基适宜外植体诱导芽萌发,最佳增殖培养基为改良MS+6-BA 4.0 mg·L^(-1)+NAA 0.5 mg·L^(-1)+TDZ 0.5 mg·L^(-1),诱导率为65.3%,最佳生根培养基为1/2MS+ABT 0.6 mg·L^(-1)+IBA 0.2 mg·L^(-1),生根率90%,活性炭浓度最佳添加浓度0.5 g·L^(-1),蔗糖最佳添加浓度30 g·L^(-1)。Using excellent tender stem segments of arbequina as experimental materials,the effects of factors such as sterilization of olive explants,basic culture medium,and growth regulators on Olea europaea tissue culture technology were studied.The experimental results showed that the highest yield 36.15% was achieved when 75% ethanol was used for 30 seconds and 0.1% HgCl_(2) 10 minutes for explant disinfection treatment.The improved MS medium was suitable for inducing shoot germination from explants.The optimal proliferation medium was the improved MS+6-BA4.0 mg·L^(-1)+NAA0.5 mg·L^(-1)+TDZ 0.5 mg·L^(-1),with an induction rate of 65.3%.The optimal rooting medium was 1/2MS+ABT 0.6 mg·L^(-1)+IBA 0.2 mg·L^(-1),with a rooting rate of 90%.The optimal concentration for adding activated carbon was 0.5 g·L^(-1),and the optimal concentration for adding sucrose was 30 g·L^(-1).

关 键 词：油橄榄 组织培养 快繁技术 

分 类 号：S565.7[农业科学—作物学]