细毡毛忍冬叶提取物的抗菌、抗氧化活性研究  

Study on antibacterial and antioxidant activities of leaves extracts from Lonicera Similis Hemsl.

作　　者：邢慧子 高平[1] 邓小宽[1] 梁歌[2] 李旭廷[2] 王斌[2] 李思聪[2] 张敏[2] XING Huizi;GAO Ping;DENG Xiaokuan;LIANG Ge;LI Xuting;WANG Bin;LI Sicong;ZHANG Min(Sichuan University,Chengdu 610000,China;Sichuan Animal Sciences Academy,Chengdu 610066,China)

机构地区：[1]四川大学,四川成都610000 [2]四川省畜牧科学研究院,四川成都610066

出　　处：《四川林业科技》2024年第4期98-105,共8页Journal of Sichuan Forestry Science and Technology

基　　金：四川省十四五重大科技专项(川猪重大疫病防控新技术新产品创制:2021ZDZX0010);国家现代农业产业技术体系四川省兽药创新团队(SCCXTD-2020-18)。

摘　　要：为代替金银花花蕾作为饲料添加剂,降低饲料成本,充分利用细毡毛忍冬叶资源,因此探究细毡毛忍冬叶粗提物、工艺优化提取物及精制提取物的抗菌、抗氧化活性。测定细毡毛忍冬叶不同提取物的主要活性成分含量,并进行各提取物对大肠杆菌、金黄色葡萄球菌、鼠寒沙门氏菌及铜绿假单胞菌的体外抑菌实验,并对抑菌效果较好的细毡毛忍冬叶精制物进行MIC(最小抑菌浓度)及MBC(最低杀菌浓度)测定。使用ABTA、DPPH、FRAP三种抗氧化能力检测方法测定各提取物总抗氧化能力。(1)细毡毛忍冬叶精制产物在抑菌活性实验中表现出显著抑菌活性,抑菌能力与金银花花蕾精制产物相近,比粗提物及工艺优化产物高。(2)细毡毛忍冬叶精制物对大肠杆菌、金黄色葡萄球菌、鼠寒沙门氏菌、铜绿假单胞菌的MIC分别为12.5 mg·mL^(-1)、6.25 mg·mL^(-1)、1.56 mg·mL^(-1)、3.125 mg·mL^(-1),MBC分别为25 mg·mL^(-1)、12.5 mg·mL^(-1)、3.125 mg·mL^(-1)、12.5 mg·mL^(-1)。(3)细毡毛忍冬叶精制物具有较强抗氧化性,其抗氧化能力随着溶液浓度的增加而逐步升高,且抗氧化性高于粗提物、工艺优化产物及金银花精制物,表明经过富集纯化的细毡毛忍冬叶产物的抗氧化性显著增加。细毡毛忍冬叶精制产物具有较好的抗菌、抗氧化活性,具有代替金银花作为饲料添加的潜力。In order to replace Lonicera japonica bud as feed additive,reduce feed cost and make full use of Lonicera similis leaves resources,the antibacterial and antioxidant activities of crude extract,optimized extract and refined extract of L.similis leaves were studied.The main active components of different extracts of L.similis leaves were determined,and in vitro antibacterial experiments of each extract against Escherichia coli,Staphylococcus aureus,Salmonella typhimurium and Pseudomonas aeruginosa were carried out,and the MIC and MBC experiments were carried out on the refined products with better antibacterial effect.The total antioxidant capacity of each extract was determined by ABTA,DPPH and FRAP.The main results are as follows:(1) The refined products of L.similis leaves showed significant antibacterial activity in the antibacterial activity experiment,and the antibacterial ability was similar to that of the flower buds of Lonicera japonica,and higher than that of the crude extract and optimized products.(2)The MIC of L.similis leaves refined products against Escherichia coli,Staphylococcus aureus,Salmonella typhimurium and Pseudomonas aeruginosa were 12.5 mg·mL^(-1),6.25 mg·mL^(-1),1.56 mg·mL^(-1) and 3.125 mg·mL^(-1),respectively,and the MBC were 25 mg·mL^(-1),12.5 mg·mL^(-1),3.125 mg·mL^(-1) and 12.5 mg·mL^(-1) respectively.(3) The L.similis leaves refined products exhibited strong antioxidant activity,and its antioxidant capacity gradually increased with the increase of solution concentration,and the antioxidant capacity was higher than that of crude extract,optimized product and refined L.japonica extract,indicating that the antioxidant capacity of L.similis Hemsl.after enrichment and purification was significantly increased.The refined product of L.similis leaves had good antibacterial and antioxidant activities,and had the potential to replace L.japonica as feed additive.

关 键 词：细毡毛忍冬叶 抗菌 抗氧化 

分 类 号：S816.7[农业科学—饲料科学]