催化剂填料表面微生物对微污染物胁迫的响应  

Response of catalyst microorganisms to the stress effects of micropollutants

作　　者：高靖雨 蔡武 梁昊 史国贵 陈友怡 吕来 邢学辞 胡春 GAO Jingyu;CAI Wu;LIANG Hao;SHI Guogui;CHEN Youyi;LYU Lai;XING Xueci;HU Chun(Key Laboratory for Water Quality and Conservation of the Pearl River Delta,Ministry of Education,Institute of Environmental Research at Greater Bay,Guangzhou University,Guangzhou 510006,China)

机构地区：[1]广州大学,大湾区环境研究院珠江三角洲水质安全与保护教育部重点实验室,广州510006

出　　处：《环境工程学报》2024年第6期1510-1519,共10页Chinese Journal of Environmental Engineering

基　　金：广东省“珠江人才计划”引进创新创业团队项目(2019ZT08L387);国家自然科学基金资助项目(52350005,52150056,51838005);广东省自然科学基金面上项目(2023A1515011509);广州市科技计划市校联合资助项目(2024A03J0088,202201020177)。

摘　　要：目前复合微污染水源条件下,饮用水中复合微污染物对微生物带来的胁迫效应会引发含氮类消毒副产物生成势(nitrogenous disinfection byproducts formation potential,N-DBPs FP)和抗性基因(antibiotic resistance genes,ARGs)水质风险。本研究基于催化剂(HCLL-S8-M)作为新型生物滤料建立反应器处理砂滤后水,考察生物滤池出水N-DBPs FP和ARGs丰度,对滤料胞外聚合物(extracellular polymeric substance,EPS)进行分析,通过宏基因组学分析水体中微生物群落结构和胁迫效应基因表达水平。结果表明,在相同复合微污染物条件下(磺胺嘧啶和2,4-二氯苯氧乙酸各1μg·L^(-1)),HCLL-S8-M滤柱出水中卤代乙腈和卤代硝基甲烷的总生成势为522.30 ng·L^(-1),比椰壳活性炭滤柱出水低171.70 ng·L^(-1),ARGs相对丰度也比椰壳活性炭滤柱出水低约4倍。这得益于HCLL-S8-M滤柱可以高效去除包括微污染物在内的溶解性有机碳、SUVA和高分子质量的有机物。HCLL-S8-M滤柱和出水中微生物分泌更少的EPS,从而减少的N-DBPs前驱体和削弱ARGs的水平转移。宏基因组学分析结果表明,微生物在HCLL-S8-M滤柱出水中的氧化应激反应功能基因表达比椰壳活性炭滤柱下降。HCLL-S8-M滤柱减弱微污染物对微生物的胁迫效应,降低N-DBPs FP和ARGs,有助于控制微生物应激反应造成间接饮用水水质风险。Under the current conditions of composite micro-polluted water sources,the stress effects of composite micropollutants in drinking water for microbes can cause water quality risks of nitrogenous disinfection byproducts formation potential(N-DBPs FP)and antibiotic resistance genes(ARGs).In this study,the catalyst HCLL-S8-M was used as a new biofilter to build the reactor treating the effluent from sand filtration.The abundances of N-DBPs FP and ARGs in the biofilter effluent were studied,the filter media and extracellular polymeric substance(EPS)was analyzed,and metagenome sequencing was used to analyze the microbial community structure and gene expression levels of stress effect in water bodies.The results showed that under the exposure of the same complex micropollutants conditions(1µg·L^(−1) of sulfadiazine and 1µg·L^(−1) of 2,4-dichlorophenoxyacetic acid),the total formation potential of haloacetonitriles and halonitromethanes was 522.30 ng·L^(−1),which was 171.70 ng·L^(−1) lower than that of coconut shell activated carbon,the relative abundance of ARGs was around 4 times lower than that of coconut shell activated carbon effluent.This was due to HCLL-S8-M could efficiently remove dissolved organic carbon(including micropollutants),SUVA and organics with high molecular weight.Microorganisms in the HCLL-S8-M filter and effluent secreted few EPS,which resulted in reduced precursors of N-DBPs and horizontal transfer of ARGs.Metagenome analysis revealed that oxidative stress functional gene expression of microorganisms decreased on HCLL-S8-M compared to coconut shell activated carbon.HCLL-S8-M attenuated the stress effects of micropollutants on microorganisms,and decreased N-DBPs FP and ARGs,which will contribute to control the indirect drinking water quality risks of micropollutants from the microbial stress response during biofiltration.

关 键 词：催化剂 微生物胁迫效应 消毒副产物 抗性基因 氧化应激反应 

分 类 号：X703[环境科学与工程—环境工程]