菌丝球强化耐盐好氧颗粒污泥构建、性能及其微生物群落特性  

Mycelial pellets enhance the construction,performance and microbial community characteristics of salt-tolerant aerobic granular sludge

作　　者：陆青青 刘勇弟[1,2] 厉巍 高会杰 王纲 LU Qingqing;LIU Yongdi;LI Wei;GAO Huijie;WANG Gang(National Engineering Research Center for Innocuous and Resourceful Industrial Wastewater,School of Resources and Environmental Engineering,East China University of Science and Technology,Shanghai 200237,China;State Key Laboratory for Environmental Risk Assessment and Control of Environmental Chemical Processes,School of Resources and Environmental Engineering,East China University of Science and Technology,Shanghai 200237,China;Chemical Industrial Zone of Shanghai Zhongfa Water Affair Devlopment Co.,Ltd.,Shanghai 201400,China;Sinopec Dalian Research Institute of Petroleum and Petrochemicals Co.,Ltd.,Dalian 116045,China)

机构地区：[1]华东理工大学资源与环境工程学院,工业废水无害化与资源化国家工程研究中心,上海200237 [2]华东理工大学资源与环境工程学院,国家环境保护化工过程环境风险评价与控制重点实验室,上海200237 [3]上海化学工业区中法水务发展有限公司,上海201400 [4]中石化(大连)石油化工研究院有限公司,大连116045

出　　处：《环境工程学报》2024年第6期1638-1651,共14页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金资助项目(52170076)。

摘　　要：基于Martelella sp.AD-3中度嗜盐功能细菌耦合活性污泥这一耐盐有机污染物强化降解体系,探究菌丝球外源投加对构建耐盐好氧颗粒污泥系统(SAGS)和PAHs类污染物去除的影响。以杭州湾潮间带污泥为接种源,在盐度为5%,以葡萄糖和菲为碳源的条件下筛选得到一株耐盐真菌Penicillium sp.L22。发现在pH为7,盐度为3%的条件下,菌丝球的生长量和降解性能都达到最佳。将上述菌丝球投加到Martelella sp.AD-3菌和活性污泥强化体系,进一步研究发现,相比于对照组(AD-3菌+活性污泥),实验组(菌丝球+AD-3菌+活性污泥)虽然造粒缓慢且形成的颗粒较小(平均粒径减小19.3%),但是形成的颗粒结构更加密实稳定,反应器生物量大(VSS增加3.2倍),污泥沉降性更好(SVI_(5)减小24.4%)。通过研究胞外多聚物(EPS)发现,菌丝球的投加增加了反应体系中污泥胞外蛋白质含量,从而促进了污泥团聚。在有机污染物降解方面,相比于对照组,菌丝球实验组在第三阶段COD去除率提升20.5%,代表性模式污染物菲的比降解活性提升53.1%。采用高通量基因测序技术分析发现,Martelella属的相对丰度与污泥的比降解活性成显著正相关,表明添加菌丝球可能是通过强化AD-3菌(Martelella属)的持留从而强化菲的降解。此外,菌丝球Penicillium属和菲的降解也成显著正相关。本研究揭示了真菌菌丝球强化SAGS系统的群落演替特征,为高盐有机废水的强化处理提供了技术支持和理论依据。based on the Martelella sp.AD-3 moderately halophilic bacteria coupled with activated sludge,an enhanced degradation system for salt-tolerant organic pollutants,the impact of exogenous mycelium pellets on the construction of a SAGS system and the removal of PAHs pollutants was explored.A salt-tolerant fungus,Penicillium sp.L22,was obtained by screening the intertidal sludge of Hangzhou Bay as the inoculation source under the conditions of 5%salinity and glucose and phenanthrene as carbon sources.It was found that under the conditions of pH 7 and salinity 3%,the growth and degradation performance of mycelial pellets reached the best.The above-mentioned mycelial pellets were added to the Martelella sp.AD-3 bacteria and activated sludge enhancement system.Further research found that compared with the control group(AD-3 bacteria+activated sludge),the experimental group(mycelial pellets+AD-3 bacteria+activated sludge)although the granulation was slow and the formed particles were small(the average particle size was reduced by 19.3%),the formed particle structure was more compact and stable,and the biomass of the reactor was large(VSS increased by 3.2 times),and the sewage Mud settlement was better(24.4%reduction in SVI5).Through the study of extracellular polymers(EPS),it was found that the addition of mycelial pellets increased the content of extracellular protein in the sludge in the reaction system,thereby promoting sludge agglomeration.In terms of the degradation of organic pollutants,compared with the control group,the removal rate of COD in the third stage of the mycelial pellets experimental group increased by 20.5%,and the specific degradation activity of the representative model pollutant phenanthrene increased by 53.1%.Using high-throughput gene sequencing technology analysis,it was found that the relative abundance of Martelella was significantly positively correlated with the specific degradation activity of sludge,indicating that the addition of mycelial pellets may enhance the persistence of AD-3 bacteri

关 键 词：耐盐好氧颗粒污泥 菌丝球 高盐有机废水 高通量基因测序技术 

分 类 号：X703[环境科学与工程—环境工程]