花生COP1基因的克隆及表达特征分析  

Cloning and expression analysis of COP1 gene in peanut

作　　者：杨永庆[1] 胡朋举 李玉荣[1] 王瑾[1] YANG Yong-qing;HU Peng-ju;LI Yu-rong;WANG Jin(Institute of Cereal and Oil Crops,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050035,China)

机构地区：[1]河北省农林科学院粮油作物研究所/河北省作物遗传育种实验室,河北石家庄050035

出　　处：《中国油料作物学报》2024年第4期819-825,共7页Chinese Journal of Oil Crop Sciences

基　　金：河北省农林科学院人才引进项目(C22R0311);国家花生现代农业产业技术体系(CARS-13);河北省现代农业产业技术体系油料创新团队(HBCT2018090101,HBCT2018090201);河北省现代种业科技创新专项(21326316D);河北省农林科学院现代农业科技创新工程(2022KJCXZX-LYS-11)。

摘　　要：为了探究花生中COP1基因的序列及表达特征,本研究从花生中克隆获得2个COP1基因,分别命名为AhyCOP1a和AhyCOP1b,以此为基础进行了相关分析。结果显示,AhyCOP1a和AhyCOP1b分别长2103 bp和2049 bp,均含有13个外显子和12个内含子。聚类结果显示AhyCOP1a和AhyCOP1b与双子叶作物的COP1基因聚成一类,且分别与AduCOP1和AipCOP1亲缘关系最近,表明AhyCOP1a和AhyCOP1b的进化分别始于花生野生种Ar⁃achis duranensis和A.ipaensis。此外,AhyCOP1a和AhyCOP1b分别有6个和7个WD40重复基序。各类物种的COP1基因在N端相似度较低,在C端WD40结构域部分相似性较高,表明WD40结构域是COP1基因的主要核心功能区域。组织表达结果显示,AhyCOP1a和AhyCOP1b在地上部的表达显著高于地下部,花中最高,其次为叶和茎。但AhyCOP1a在地下组织的表达量在显著高于AhyCOP1b,暗示AhyCOP1a在地下组织发育过程中的作用更大。此外还发现光照可以促进花生COP1基因的表达,表明花生中COP1基因的表达受光照显著影响。总之,本研究结果初步明确了花生中COP1基因的序列及表达特征,为后续深入探究COP1基因的分子功能奠定了基础。To explore the sequence and expression pattern of COP1 gene in peanut,two COP1 genes were cloned from peanut,named as AhyCOP1a and AhyCOP1b,and the related analysis were conducted in this study.The results showed that AhyCOP1a and AhyCOP1b were 2103 bp and 2049 bp in length,respectively,and both of them contained 13 exons and 12 introns.The phylogenetic results showed that AhyCOP1a and AhyCOP1b grouped with COP1 genes in dicotyledon crops,and were closely related to AduCOP1 and AipCOP1,respectively.The results indicated that the evolution of AhyCOP1a and AhyCOP1b originated from peanut wild accessions Arachis duranensis and A.ipaensis,respectively.In addition,AhyCOP1a and AhyCOP1b had 6 and 7 WD40 repeat motifs,respectively.The similarity of COP1 gene in N-terminus is low,and the homology of WD40 domain in C-terminus is high,indicating that WD40 domain is the main core functional region of COP1 gene.The tissue expression results showed that the expression pattern of AhyCOP1a and AhyCOP1b in the aboveground part was significantly higher than that in the underground part,and the highest expression was found in flowers,followed by leaves and stems.However,the expression level of AhyCOP1a in underground tissues is significantly higher than that of AhyCOP1b,suggesting that AhyCOP1a plays a more important role in the development of underground tissues.In addition,it was also found that light can promote the expression of COP1 gene in peanut,indicating that the expression of COP1 gene in peanut is significantly affected by light.In conclusion,the results of this study preliminarily clarified the sequence and expression pattern of COP1 gene in peanut,which laid a foundation for further exploration of the molecular function of COP1 gene.

关 键 词：花生 COP1 序列比对 表达特征 

分 类 号：S565.2[农业科学—作物学]