大豆株高全基因组关联分析  

Genome-wide association analysis of plant height in soybean

作　　者：张威 许亚男 许文静[1,2] 张红梅 刘晓庆[1] 王琼[1] 崔晓艳[1] 陈新[1] 王显生[3] 陈华涛[1] ZHANG Wei;XU Ya-nan;XU Wen-jing;ZHANG Hong-mei;LIU Xiao-qing;WANG Qiong;CUI Xiao-yan;CHEN Xin;WANG Xian-sheng;CHEN Hua-tao(Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;Nanjing Agricultural University,Nanjing 210095,China;Institute of Crop Germplasm and Biotechnology,Jiangsu Academy of Agricultural Sciences/Nanjing Station for DUS Testing Center of New Varieties of Plants of MARA,Nanjing 210014,China)

机构地区：[1]江苏省农业科学院经济作物研究所,江苏南京210014 [2]南京农业大学,江苏南京210095 [3]江苏省农业科学院种质资源与生物技术研究所/农业农村部植物新品种测试南京分中心,江苏南京210014

出　　处：《中国油料作物学报》2024年第4期826-832,共7页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(32201845、32001455);江苏省农业科技自主创新项目(CX(21)3117、CX(22)5002);国家重点研发计划(2018YFE0112200);江苏省重点研发项目(BE2019376);江苏省自然科学基金项目(BK20210154);江苏种子振兴揭榜挂帅项目(JBGS[2021]060)。

摘　　要：以包含264份种质材料的大豆自然群体为研究对象,调查该群体2019年和2020年的大豆株高性状,并进行全基因组关联分析(Genome-wide association study,GWAS),解析大豆株高遗传机制。研究发现大豆群体株高变异丰富,呈连续分布状态,广义遗传力为67.38%,同时检测到91个SNP与大豆株高显著关联,分别位于第3、4、6和19号染色体上。其中位于19号上的显著关联信号是一个控制大豆株高的主效位点,可以在2年环境中检测到,表型变异解释率最高达12.19%。对该位点内的候选基因进行筛选获得5个调控大豆株高候选基因,其中GmDt1可能是这个位点的效应基因。除此以外,在3号和6号染色体上筛选得到3个调控大豆株高的候选基因。本研究结果为大豆株高遗传改良提供了新的基因资源和参考依据。To investigate the genetic mechanism of soybean plant height,genome wide association study(GWAS)was carried out to detect the SNP significantly associated with plant height in natural population containing 264 soybean germplasm resources in 2019 and 2020.There is extremely genetic variation in the plant height of soybean population,showing continuously distribution,and the broad sense heritability was 67.38%.It was found that 91 SNPs were detected to be significantly associated with soybean plant height,which were located on chromosomes 3,4,6 and 19,respectively.Among them,the significant correlation signal on Chr.19 is a major QTL controlling soybean plant height,which was detected in both 2019 and 2020,which explained 12.19%of the phenotypic variance.At the same time,five candidate genes for regulating soybean plant height were obtained in this locus,of which Glyma.19G194300(GmDt1)maybe is the causal gene for this major QTL.In addition,three candidate genes for regulating soybean plant height were identified on chromosome 3 and 6.The results of this study provide new genetic resources and important reference for the genetic improvement of soybean plant height.

关 键 词：大豆 株高 全基因组关联 

分 类 号：S565.4[农业科学—作物学]