高效液相色谱法检测水环境中全氟辛酸铵的研究  

Study on Detection of Ammonium Perfluorooctanoate in the Water Environment by High Performance Liquid Chromatography

作　　者：梁佳童 薛英文[1] 朱玉华 LIANG Jia-tong;XUE Ying-wen;ZHU Yu-hua(School of Civil Engineering,Wuhan University,Wuhan 430072,Hubei Province,China)

机构地区：[1]武汉大学土木建筑工程学院,湖北武汉430072

出　　处：《中国农村水利水电》2024年第8期54-59,66,共7页China Rural Water and Hydropower

基　　金：湖北省自然科学基金项目(2022CFB057)。

摘　　要：全氟辛酸铵(APFO)是一种难降解、生物积累和有毒的全氟和多氟烷基化合物(PFAS),对环境和生物健康构成严重威胁。虽然质谱法对PFAS的检测效果较好,但其成本高昂。高效液相色谱(HPLC)法是一种成本较低的检测方法,但现有的技术稳定性、重现性和效率较低。研究成功建立了一种以高氯酸水溶液和乙腈为流动相能够分离和检测APFO的HPLC法,系统探究了流动相体积比和pH对APFO检测的影响。结果表明,在高氯酸水溶液∶乙腈=60∶40,pH=3.5条件下呈现出最佳的APFO的出峰效果。该方法单样耗费时间少于15 min,检测限为1.01 mg/L,使用更简便的操作和花费更少的时间与成本达到了与其他HPLC方法相近的灵敏度,并在2~50 mg/L的浓度范围具有良好的线性。精密度和准确度实验结果表明,本方法相对标准偏差在9%以内,对实际水体的加标回收率在97.35%~104.80%之间,表现出对复杂样品测定的抗干扰性。本研究展示了该方法在水处理材料开发初期的实验环境中高效且经济地检测高浓度PFAS的巨大潜力。Ammonium Perfluorooctanoate(APFO)is a hard-to-degrade,bioaccumulative and toxic per-and polyfluoroalkyl substances(PFAS)that poses a serious threat to the environment and biological health.Although mass spectrometry is effective in detecting PFAS,it is expensive.High-performance liquid chromatography(HPLC)method is a lower-cost detection method,but the existing technology has low stability,reproducibility and efficiency.This study successfully established an HPLC method with aqueous perchloric acid and acetonitrile as mobile phases capable of separating and detecting APFO.The study systematically investigated the effects of mobile phase volume ratio and pH on the detection of APFO.The results showed that the best APFO peaks were obtained under the conditions of aqueous perchloric acid∶acetonitrile=60∶40,pH=3.5.This method consumed less than 15 minutes per sample,and the limit of quantification of the method was 1.01 mg/L.This method achieved similar sensitivity to other HPLC methods using simpler operation and less time and cost,and showed good linearity in the concentration range of 2~50 mg/L.The precision and accuracy experiments showed that the relative standard deviation of the method was within 9%,and the spiked recoveries were between 97.35%and 104.80%for actual water samples,which demonstrated anti-interference for determining complex samples.This study showcases the method′s significant potential for efficient and economical detection of high-concentration PFAS in water treatment material development during initial stages.

关 键 词：高效液相色谱法 紫外检测 全氟辛酸铵 全氟化合物 全氟检测 

分 类 号：TV41[水利工程—水工结构工程] X83[环境科学与工程—环境工程]