低成本共聚焦荧光检测器的研制及其对水样中铵氮测定  

Development of Low-Cost Confocal Fluorescence Detector and Its Application to Determination of Ammonium Nitrogen in Water Samples

作　　者：张政 陈学佳 曾梓涵 张敏 ZHANG Zheng;CHEN Xuejia;ZENG Zihan;ZHANG Min(School of Life and Environmental Sciences,Guilin University of Electronic Technology,Guilin 541004,Guangxi China)

机构地区：[1]桂林电子科技大学生命与环境科学学院,广西桂林541004

出　　处：《分析测试技术与仪器》2024年第4期215-220,共6页Analysis and Testing Technology and Instruments

基　　金：国家自然科学基金(Nos.22174028,21966012)。

摘　　要：研究利用3D打印技术研制了一台低成本共聚焦荧光检测器,并将其用于检测河水水样中的铵氮.检测器采用共聚焦结构,使用波长为430 nm的发光二极管(LED)光源、球透镜和平凸透镜组成的准直透镜组以及滤光片等光学元件.研究通过3D打印技术制作各种模块,用于固定各光学元件.合成了喹啉-2,3-二甲醛(QDA)作为铵氮荧光检测试剂.QDA试剂与NH_(4)^(+)-SO_(3)^(2-)-Ca^(2+)反应,其产物的最大激发波长为429 nm,最大发射波长为518 nm.采用研制的检测器以及合成的QDA试剂建立铵氮荧光检测方法,方法检测限为0.038μmol/L(3σ),平行测定浓度为3.0μmol/L的铵氮标准溶液,其相对标准偏差(RSD)为2.21%.应用于实际水样检测时,加标回收率在89.8%~93.7%之间.通过使用3D打印技术辅助检测器的研制,降低了制作成本,同时实现了对结构的快速优化.研制的检测器稳定性好、灵敏度高、结构简单,可应用于环境、食品等领域的样品快速检测.A low-cost confocal fluorescence detector was developed using 3D printing technology and applied to the determination of ammonium nitrogen in river water samples.The detector used a confocal structure,and utilized lightemitting diode(LED)with a wavelength of 430 nm as light source,along with optical components such as a ball lens,plano-convex lens forming a collimating lens and filters.Various modules were produced by 3D printing technology for fixing various optical components.Quinoline-2,3-dicarbaldehyde(QDA)was synthesized as the fluorescent reagent for ammonium nitrogen detection.QDA reacts with NH_(4)^(+)-SO_(3)^(2-)-Ca^(2+),and the product has a maximum excitation wavelength of 429 nm and maximum emission wavelength of 518 nm.The developed detector,along with the synthesized QDA reagent were used to establish a fluorescence detection method for ammonium nitrogen.The limit of detection of the method was 0.038μmol/L(3σ),and the relative standard deviation(RSD)was 2.21%for detecting 3.0μmol/L ammonium nitrogen standard solution.The spiked recoveries ranged from 89.8%to 93.7%when applied to the detection of river water samples.Leveraging 3D printing technology facilitated the development of the detector,the manufacturing costs were reduced and the rapid optimization of the structure was enabled.The developed detector demonstrates excellent stability,high sensitivity,and a simple structure,making it suitable for rapid sample testing in environmental and food analysis applications.

关 键 词：3D打印 荧光检测器 喹啉-2 3-二甲醛 共聚焦 

分 类 号：O657.39[理学—分析化学] TH74[理学—化学]