各向异性PCL/明胶复合人工心脏瓣膜瓣叶材料的制备及性能  

Preparation and properties of anisotropic PCL/gelatin composite artificial heart valve leaflet materials

作　　者：陈昱欣 王雅娟 郑小凡 王富军[1,2] 王璐[1,2] 侯永泰 王盛章 李超婧 Chen Yuxin;Wang Yajuan;Zheng Xiaofan;Wang Fujun;Wang Lu;Hou Yongtai;Wang Shengzhang;Li Chaojing(College of Textiles,Donghua University,Shanghai 201620,China;Key Laboratory of Textile Science&Technology,Ministry of Education,Donghua University,Shanghai 201620,China;Institute of Biomedical Engineering and Technology,Academy for Engineering and Technology,Fudan University,Shanghai 200433,China;Institute of Biomechanics Department of Aeronautics and Astronautics,Fudan University,Shanghai 200433,China;Shanghai Haohai Biological Technology Co.,Ltd.,Shanghai 200052,China)

机构地区：[1]东华大学纺织学院,上海201620 [2]东华大学纺织面料技术教育部重点实验室,上海201620 [3]复旦大学工程与应用技术研究院生物医学工程技术研究所,上海200433 [4]复旦大学航空航天系生物力学研究所,上海200433 [5]上海昊海生物科技股份有限公司,上海200052

出　　处：《产业用纺织品》2024年第7期1-10,共10页Technical Textiles

基　　金：中国博士后科学基金(2023M732187);中央高校基本科研业务费专项资金资助(2232023G-01);111计划2.0(BP0719035)。

摘　　要：为制备模拟人体原生瓣膜层状结构及力学各向异性的人工心脏瓣膜瓣叶材料,采用静电纺丝技术,以不同质量配比的聚己内酯(PCL)和明胶为原料,通过调整各层纤维膜的纤维排列情况,顺序堆叠纺制3层结构的PCL/明胶复合支架。结果表明,PCL质量体积比为10%时,随着明胶质量分数增加,纤维直径和复合支架厚度随之增加,复合支架的弹性模量和极限抗拉强度减小。其中,PCL和明胶质量配比为5∶3的复合支架在两个相互垂直方向上的弹性模量分别为(26.98±3.01)MPa和(3.39±0.36)MPa,各向异性比约为7.96,接近原生主动脉瓣的力学各向异性比,其体外脉动流测试显示有效开口面积(EOA)为1.79 cm^(2)、平均跨瓣压差(MPD)为1.67 kPa(即12.52 mmHg)、反流分数(RF)为24.22%。同时,制备的复合支架溶血率均小于5%,细胞相对增殖率均大于80%,具有良好的血液相容性和细胞相容性,有望应用于人工心脏瓣膜瓣叶材料。To fabricate artificial heart valve leaflet materials that mimic the layered structure and mechanical anisotropy characteristics of human native heart valve leaflets,different mass ratios of polycaprolactone(PCL)and gelatin were used to produce PCL/gelatin composite scaffolds by electrospinning technology.The three-layered composite scaffolds were stacked sequentially by adjusting the fiber arrangement of each layer membrane.The results showed that with a PCL mass-volume ratio of 10%,as the gelatin mass fraction increased,the fiber diameter and the thickness of the composite scaffolds increased,while the elastic modulus and ultimate tensile strength of the scaffolds decreased.Specifically,the elastic modulus of the composite scaffold with a mass ratio of PCL and gelatin of 5∶3 was(26.98±3.01)MPa and(3.39±0.36)MPa in two mutually perpendicular directions,respectively,with an anisotropy ratio of approximately 7.96,approaching the mechanical anisotropy ratio of native aortic valve leaflets.In vitro pulsatile flow experiments showed that the effective opening area(EOA)was 1.79 cm^(2),the mean pressure difference(MPD)was 1.67 kPa(12.52 mmHg)and the regurgitant fraction(RF)was 24.22%.Meanwhile,the hemolysis rate of the prepared composite scaffolds was less than 5%,and the relative cell proliferation rate exceeded 80%,indicating good hemocompatibility and cytocompatibility,which suggested potential application in artificial heart valve leaflet materials.

关 键 词：人工心脏瓣膜 各向异性 静电纺丝 层状结构 弹性模量 极限抗拉强度 溶血率 有效开口面积(EOA) 平均跨瓣压差(MPD) 反流分数(RF) 

分 类 号：R318.11[医药卫生—生物医学工程] TS176[医药卫生—基础医学]