异基因造血干细胞移植后宏基因组二代测序临床诊断耶氏肺孢子菌肺炎的单中心临床分析  

Single center clinical analysis of metagenomic next-generation sequencing for clinical diagnosis of Pneumocystis jirovecii Pneumonia after allogeneic hematopoietic stem cell transplantation

作　　者：周洋[1] 陈刚[1] 韩朋 饶冠华 徐建丽[1] 张凯乐 杨蕊雪 江明[1] 袁海龙[1] ZHOU Yang;CHEN Gang;HAN Peng;RAO Guanhua;XU Jianli;ZHANG Kaile;YANG Ruixue;JIANG Ming;YUAN Hailong(Department of Hematology,The First Affiliated Hospital of Xinjiang Medical University,Urumqi,830000,China;Tianjin Genskey Medical Technology Co.,Ltd CN)

机构地区：[1]新疆医科大学第一附属医院血液科,乌鲁木齐830000 [2]天津金匙医学科技有限公司

出　　处：《临床血液学杂志》2024年第7期504-511,共8页Journal of Clinical Hematology

基　　金：“天山英才”医药卫生高层次人才培养计划(No:TSYC202301B022)。

摘　　要：目的:探讨异基因造血干细胞移植(allogeneic hematopoietic stem cell transplantation,allo-HSCT)后经宏基因组二代测序(metagenomic next-generation sequencing,mNGS)协助诊断的耶氏肺孢子菌肺炎(Pneumocystis jirovecii pneumonia,PJP)患者的临床主要特征。方法:2020年10月—2023年3月allo-HSCT后的19例发热患者,完善常规微生物学检测及影像学检查无法明确诊断,经验性抗感染治疗无效时,通过mNGS检测临床诊断为PJP。分析患者的主要临床表现,mNGS结果、细胞免疫功能、炎症因子、血气分析、G-试验等,肺部高分辨CT、支气管镜结果以及治疗和转归等临床特征。结果:Allo-HSCT后PJP发生的中位时间为6个月,发热和呼吸困难是PJP的主要临床症状。19例患者均出现发热,以持续中度发热为主,安静状态下呼吸困难患者10例,3例患者为活动后出现呼吸困难;CD4+T淋巴细胞计数的中位值为148(34~394)个/μL,其中12例CD4+T淋巴细胞计数<200个/μL,10例患者血气分析提示Ⅰ型呼吸衰竭,氧分压的中位值为59.5(36.8~74.0)mmHg(1 mmHg=0.133 kPa),双变量相关分析显示CD4+T淋巴细胞计数与患者氧分压呈正相关(r=0.468,P=0.043)。9例(47.36%)PJP患者G试验阳性;肺部CT主要以多发磨玻璃样密度增高影为主;17例PJP患者行支气管镜并肺泡灌洗液(BALF)检测mNGS,耶氏肺孢子菌核酸序列数的中位数为487(178~131444)条,中位相对丰度为56.9%(26.0%~99.5%),双变量相关分析显示患者氧分压与PJP序列数呈显著负相关(r=-0.498,P=0.042)。2例患者行外周血耶氏肺孢子菌核酸序列数分别为2条和4条;19例患者中12例发生PJP时存在慢性移植物抗宿主病,13例患者发生PJP前接受剂量不等的糖皮质激素治疗。诊断PJP后,患者主要接受复方磺胺甲噁唑(SMZ)/甲氧苄啶(TMP)联合卡泊芬净或米卡芬净治疗,1例患者因呼吸功能衰竭死亡,其余患者均治愈。结论:Allo-HSCT后发生PJP的患者主要以发热和呼吸困难为�Objective To explore the clinical characteristics of Pneumocystis jirovecii Pneumonia(PJP)patients diagnosed with metagenomic next-generation sequencing(mNGS)assisted by allogeneic hematopoietic stem cell transplantation(allo-HSCT).Methods From October 2020 to March 2023,19 patients with fever were diagnosed with PJP through mNGS testing after allo-HSCT at our transplantation center,as routine microbiological and imaging examinations were unable to determine the diagnosis,and empirical anti-infective treatment was ineffective.The main clinical manifestations of the patients were analyzed,including mNGSresults,cellular immune function,inflammatory factors,blood gas analysis,G-experiments,high-resolution CT and bronchoscopyresults of the lungs,as well as clinical features such as treatment and prognosis.Results The median time of occurrence of PJP after allo-HSCT was 6 months,and fever and dyspnea were the main clinical symptoms of PJP.All 19 patients experienced fever,with persistent moderate fever being the main cause.10 patients had difficulty breathing in a quiet state,and 3 patients had difficulty breathing after activity.The average CD4+T lymphocyte count was 148(34-394)/μL.Among them,12 cases had CD4+T lymphocyte counts<200/μL.Blood gas analysis of 10 patients showed type I respiratory failure,with an average oxygen partial pressure of 59.5(36.8-74.0)mmHg.Bivariate correlation analysis showed a positive correlation between CD4+T lymphocyte count and patient oxygen partial pressure(r=0.468,P=0.043).9 PJP patients(47.37%)showed positive G-testresults;The CT imaging of the lungs mainly shows multiple ground glass like high-density shadows;17 PJP patients underwent bronchoscopy and alveolar lavage fluid(BALF)to detect mNGS.The median nucleic acid sequence number of Pneumocystis jirovecii Pneumonia was 487(178-131444),and the median relative abundance was 56.9%(26.0%-99.5%).Bivariate correlation analysis showed a significantly negative correlation between patient oxygen partial pressure and PJP sequence number(

关 键 词：异基因造血干细胞移植 耶氏肺孢子菌肺炎 宏基因组二代测序 

分 类 号：R457.7[医药卫生—治疗学]