垂黄清脉饮对再狭窄模型兔的抗血小板活化的作用  

Effect of Chui Huang Qing Pulse Drink on Platelet Activation in a Restenosis Rabbit Model

作　　者：金学连 何霄 吴丹 李建红[3] 赵凯[3] JIN Xue-lian;HE Xiao;WU Dan;LI Jian-hong;ZHAO Kai(School of Traditional Chinese Medicine,Ningxia Medical University,Yinchuan Ningxia 750004,China;Key Laboratory of Modern Education for Nationalities,Ministry of Education,Ningxia Medical University,Yinchuan Ningxia 750004,China;General Hospital of Ningxia Medical University,Yinchuan Ningxia 750004,China)

机构地区：[1]宁夏医科大学中医学院,宁夏银川750004 [2]宁夏少数民族现代化教育部重点实验室,宁夏银川750004 [3]宁夏医科大学总医院,宁夏银川750004

出　　处：《时珍国医国药》2024年第6期1312-1315,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(8186150472);中国中医科学院中医基础理论研究所合作单位个人项目(YZ-202129)。

摘　　要：目的观察垂黄清脉饮对再狭窄的血小板活化的影响,进而探讨其治疗再狭窄的作用机理。方法选取70只新西兰大白兔,随机分为空白组,模型组,阿托伐他汀组、垂黄清脉饮低、中、高浓度组,垂黄清脉饮联合阿托伐他汀组。9周造模完成后,分别用对应药物灌对应组兔,灌胃的第1、2、3、4周于耳缘静脉取血,酶联免疫吸附试验法(ELSA)检测血清中血栓素B2(TXB2)和6-酮前列腺素F1α(6-Keto-PGF1α)的指标,采用兽用B超及HE染色观察造模效果及治疗变化。结果对比空白组,模型组兔血清中TXB2显著升高(P<0.01),6-Keto-PGF1α则显著减少(P<0.01);对比模型组,随着灌胃时间的变化,各用药组的TXB2逐渐降低、6-Keto-PGF1α逐渐升高,其中垂黄清脉饮高浓度组和垂黄清脉饮联合阿托伐他汀组均显著变化(P<0.001);对比阿托伐他汀组,随着时间变化,中药组和中药联合西药组均有差异(P<0.05),且灌胃时间越久,中药浓度越高、中药联合阿托伐他汀对血清TXB2和6-Keto-PGF1α影响越大(P<0.01)。结论垂黄清脉饮对兔再狭窄具有抗血小板活化的作用,且与阿托伐他汀联合后具有协同效应,可能与调控血小板聚集作用的相关因子TXB2和6-Keto-PGF1α有关,且这种作用具有时间依赖性和浓度依赖性。Objective To observe the effect of Chui Huang Qing Pulse Drink on platelet activation in restenosis and explore its mechanism of treating restenosis.Methods Seventy New Zealand white rabbits were randomly divided into the blank group,model group,atorvastatin group,low,medium,and high concentrations of Chui Huang Qing Pulse Drink groups,and Chui Huang Qing Pulse Drink combined with atorvastatin group.After 9 weeks of modeling,corresponding drugs were orally administered to the corresponding groups of rabbits.Blood samples were collected from the ear vein in the first,second,third,and fourth week,and the levels of thromboxane B2(TXB2)and 6-keto-prostaglandin F1α(6-Keto-PGF1α)in the serum were measured by enzyme-linked immunosorbent assay(ELISA).The modeling effect and therapeutic changes were observed by veterinary ultrasound and HE staining.Results Compared with the blank group,the serum TXB2 level in the model group was significantly increased(P<0.01),while 6-Keto-PGF1αlevel was significantly decreased(P<0.01).Compared with the model group,with the change of oral administration time,TXB2 gradually decreased and 6-Keto-PGF1αgradually increased in each treatment group.The high concentration of Chui Huang Qing Pulse Drink group and Chui Huang Qing Pulse Drink combined with atorvastatin group showed significant changes(P<0.001).Compared with the atorvastatin group,there were differences in the traditional Chinese medicine group and the traditional Chinese medicine combined with western medicine group with the change of time(P<0.05),and the longer the oral administration time,the higher the concentration of traditional Chinese medicine and the greater the effect on serum TXB2 and 6-Keto-PGF1α(P<0.01).Conclusion Chui Huang Qing Pulse Drink has an inhibitory effect on platelet activation in rabbit restenosis,and it has a synergistic effect when combined with atorvastatin.This may be re-lated to the regulation of platelet aggregation-related factors TXB2 and 6-Keto-PGF1α,and the effect is dependent on time and conc

关 键 词：再狭窄 垂黄清脉饮 血小板活化 血栓素B2 6-酮前列腺素F1Α 

分 类 号：R285.5[医药卫生—中药学]