基于HSP70探讨中医刮痧对腰椎间盘突出症大鼠皮肤组织的影响  

Exploring the effects of traditional Chinese medicine Guasha on the skin tissues of rats with lumbar intervertebral disc herniation based on HSP70

作　　者：王佳怡 高玉洁 徐语晨 葛浩天 王庆[1] 杨敏[1] 姜荣荣[1] 徐桂华[1] WANG Jia-yi;GAO Yu-jie;XU Yu-chen;GE Hao-tian;WANG Qing;YANG Min;JIANG Rong-rong;XU Gui-hua(Nanjing University of Chinese Medicine,Nanjing,Jiangsu 210023 China)

机构地区：[1]南京中医药大学,江苏南京210023

出　　处：《时珍国医国药》2024年第6期1506-1511,共6页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金面上项目(81473791);江苏省高等学校基础科学(自然科学)面上项目(21KJB360024);2023年江苏省研究生科研创新计划(KYCX23_2197)。

摘　　要：目的以腰椎间盘突出症(LDH)模型大鼠为观察对象,观察刮痧对大鼠的镇痛作用,探讨其是否通过促进热休克蛋白70(HSP70)的表达来影响皮肤络脉,促进局部皮肤血液循环,从而缓解脉络闭阻,调控背根神经节损伤达到镇痛效果。方法以40只SD雄性大鼠为研究对象,随机分为假手术组、模型组、刮痧组及假刮痧组,每组10只。采用自体髓核移植建立LDH疾病模型,给予督脉和膀胱经刮痧干预,观察刮痧对其右后足机械痛阈值(PWT)、局部皮肤温度、血清转化生长因子-β1(TGF-β1)、背根神经节(DRG)和背部局部皮肤形态学、局部皮肤组织HSP70 mRNA及蛋白的影响。结果与模型组相比,刮痧组PWT明显升高(P<0.05),但假刮痧组无统计学差异(P>0.05);较假手术组、模型组和假刮痧组等非刮痧组相比,刮痧后大鼠背部局部皮肤温度显著升高(P<0.05);与假手术组相比,模型组大鼠血清TGF-β1水平明显降低(P<0.05),刮痧组TGF-β1水平明显高于模型组和假刮痧组(P<0.05);与假手术相比,模型组和假刮痧组大鼠的DRG形态显示出明显的炎性水肿和损伤迹象,而刮痧组神经损伤和炎性浸润情况减轻;与假手术组、模型组和假刮痧组等非刮痧组相比,刮痧组皮肤真皮层可见毛细血管扩张,分布丰富的红细胞,部分浸润中性粒细胞和淋巴细胞等免疫细胞;较假手术组、模型组和假刮痧组相比,刮痧组大鼠皮肤组织中HSP70 mRNA和蛋白表达均显著增加(P<0.05)。结论刮痧可减轻机体炎症反应,缓解LDH引发的疼痛反应,其机制可能与升高局部皮肤温度,刺激皮肤络脉,调动机体级联反应,促进局部皮肤血液循环,缓解脉络闭阻引起的瘀滞有关,其中HSP70蛋白可能是刮痧激活机体级联反应的关键靶点蛋白。Objective The analgesic effect of Guasha on rats was observed in lumbar disc herniation(LDH)model rats to explore whether it affects the skin chakra and promotes local skin blood circulation by promoting the expression of HSP70,thus relieving the chakra occlusion and regulating dorsal root ganglion injury to achieve analgesic effect.Methods Forty SD male rats were randomly divided into sham group,model group,Guasha group and sham-Guasha group,with 10 rats in each group.Autologous myeloid transplantation was used to establish the LDH disease model,and Guasha intervention was given to the Dumai and bilateral bladder meridian to observe its effects on the mechanical pain threshold(PWT)of their right hindfoot,local skin temperature,serum TGF-β1,dorsal root ganglion(DRG)and local skin morphology of the back,and the local skin tissues of HSP70 mRNA and proteins.Results Compared with the model group,PWT was significantly higher in the Guasha group(P<0.05),but there was no statistically significant difference in the sham-Guasha group(P>0.05);compared with the non-Guasha groups,such as the sham group,the model group,and the sham-Guasha group,local skin temperature of the back of the rats after Guasha was significantly higher(P<0.05);compared with the sham group,serum TGF-β1 level of the model group was significantly lower(P<0.05),the TGF-β1 level in the gua sha group was significantly higher than that in the model and sham-Guasha groups(P<0.05);the DRG morphology of the rats in the model and sham-Guasha groups showed obvious signs of inflammatory edema and injury compared to the sham group,whereas the nerve injury and inflammatory infiltration in the Guasha group were reduced;capillary dilatation was visible in the dermal layer of the skin of the Guasha group compared to the non-gua sha groups.The dermis of the skin in the Guasha group showed dilated capillaries,scattered distribution of abundant erythrocytes,and partial infiltration of immune cells such as neutrophils and lymphocytes;compared with the non-Guasha group

关 键 词：刮痧 刮痧疗法 HSP70 热休克蛋白70 腰椎间盘突出症 镇痛 

分 类 号：R244.5[医药卫生—针灸推拿学]