基于MDR模型的喹诺酮类抗生素对蛋白核小球藻联合毒性作用评估  

Joint Toxicity Evaluation of Quinolones Drugs on Chlorella pyrenoidosa Based on MDR Model

作　　者：闫嘉宁 孟庆俊[1] 魏海生 李琳 郝研宁 YAN Jianing;MENG Qingjun;WEI Haisheng;LI Lin;HAO Yanning(School of Environmental Science and Spatial Informatics,China University of Mining and Technology,Xuzhou 221116,China)

机构地区：[1]中国矿业大学环境与测绘学院,江苏徐州221116

出　　处：《环境科学研究》2024年第8期1846-1857,共12页Research of Environmental Sciences

基　　金：江苏省环保科研课题基金(No.2018002)。

摘　　要：为考察新污染物中喹诺酮类抗生素混合物的联合毒性效应,以常见的氧氟沙星(Ofloxacin,OFXL)、环丙沙星(Ciprofloxacin,CIP)、诺氟沙星(Norfloxacin,NFXL)3种喹诺酮类抗生素为供试品,通过96 h急性毒性试验测定3种抗生素对蛋白核小球藻(Chlorella pyrenoidosa)生长抑制的单一毒性及二元和三元混合体系(共计20组)的联合毒性,通过等效线图法、浓度加和模型(CA)、独立作用模型(IA)、模型偏移率(MDR)和组合指数法评价二元和三元混合体系抗生素的相互作用类型。结果表明:①3种喹诺酮类抗生素对蛋白核小球藻在96 h均呈现明显的生长抑制,以半数效应浓度EC_(50)的负对数(pEC_(50))作为判断毒性大小的指标,毒性呈NFXL(pEC_(50)=-2.02)>CIP(pEC_(50)=-2.12)>OFXL(pEC_(50)=-2.23)的特征。②混合体系毒性相互作用类型以协同作用为主,毒性相互作用类型与混合体系组分浓度比、毒性效应区域和污染物浓度范围有关。混合体系中混合物组分浓度比最接近1∶1时,协同作用最强,毒性最大。③混合体系在低浓度范围(0%~30%)时,毒性相互作用类型以加和作用为主;在中高浓度范围(30%~100%)时,毒性相互作用随混合体系的不同而呈现出不同类型。研究显示,环境水体中喹诺酮类抗生素对蛋白核小球藻有一定的毒性效应,且抗生素二元、三元混合体系的协同毒性效应呈毒性增大的趋势。In order to study the combined toxicity of quinolone antibiotic mixtures on novel pollutants,three common quinolone antibiotics,including ofloxacin(OFXL),ciprofloxacin(CIP),and norfloxacin(NFXL),were used as experimental materials.The combined toxicity of three antibiotics on growth inhibition of Chlorella pyrenoidosa in a single,binary and ternary mixed systems(20 groups)was determined using a 96 hour acute toxicity assay.The types of antibiotic interactions in binary and ternary mixed systems were evaluated using equilibrium line graph method,concentration addition and summation(CA)model,independent action model(IA),model deflection rate(MDR)and combination index method.The results showed that the three quinolone antibiotics had a significant growth inhibitory effect on Chlorella pyrenoidosa at 96 hours.Using the negative logarithm pEC_(50) of EC_(50) as the toxicity indicator,the toxicity order of the three antibiotics is NFXL(pEC_(50)=-2.02)>CIP(pEC_(50)=-2.12)>OFXL(pEC_(50)=-2.23).The main type of toxicity interaction in mixed systems is synergistic effect.The type of toxic interaction is related to the concentration ratio of each component in the mixed systems,the toxic effect area,and the concentration range of pollutants.When the concentration ratio of the components in the mixed system is closest to 1∶1,the synergistic effect is strongest and the toxicity is highest.The low concentration range(0%-30%)of the hybrid system is mainly dominated by additive interactions,while the types of toxic interactions in the medium to high concentration range(30%-100%)vary depending on the different hybrid systems.The acute toxicity test results indicate that quinolone antibiotics in environmental water have a certain toxic effect on Chlorella pyrenoidosa,and the synergistic toxic effect of antibiotic binary and ternary mixtures shows an increasing trend in toxicity.

关 键 词：喹诺酮类抗生素 联合毒性效应 浓度加和模型 模型偏移率(MDR) 

分 类 号：X592[环境科学与工程—环境工程]