三叶青叶总黄酮的纯化工艺  

Purification technology of total flavonoids from Tetrastigma hemsleyanum

作　　者：傅长泳 许海顺 FU Changyong;XU Haishun(Zhejiang Agronomist College,Hangzhou 310021,Zhejiang;Zhejiang A&F University,Hangzhou 311300,Zhejiang)

机构地区：[1]浙江农艺师学院,浙江杭州310021 [2]浙江农林大学,浙江杭州311300

出　　处：《浙江农业科学》2024年第8期1759-1763,共5页Journal of Zhejiang Agricultural Sciences

基　　金：遂昌县县校合作科技计划(SCX2024HZ20);浙江省公益技术研究计划(LGG21H280001);浙江省林业科研推广项目(2020B06)。

摘　　要：为优化聚酰胺分离纯化三叶青叶总黄酮的工艺条件,采用紫外分光光度法测定三叶青叶总黄酮的含量,通过聚酰胺树脂对黄酮进行吸附,不同处理后从静态和动态吸附以及解吸性能3个方面展开研究。结果表明:聚酰胺树脂吸附三叶青叶总黄酮的最佳条件是上样液浓度15 mg·mL^(-1),聚酰胺树脂1.75 g,并将pH值控制在3.0左右进行静态吸附,最高吸附率为76.7%。之后使用60%的乙醇将聚酰胺树脂进行解吸,解吸率最高为75.9%。In order to optimize the process conditions for the separation and purification of total flavonoids from polyamide,the content of total flavonoids in Tetrastigma hemsleyanum was determined by ultraviolet spectrophotometry,and the flavonoids were adsorbed by polyamide resin,and the static and dynamic adsorption and desorption properties were studied from three aspects:static and dynamic adsorption and desorption performance after different treatments.The results showed that the optimal conditions for the adsorption of total flavonoids in the leaves of Tetrastigma hemsleyanum were 15 mg·mL^(-1) and 1.75 g of polyamide in the loading solution,and the pH value was controlled at about 3.0 for static adsorption,and the highest adsorption rate was 76.7%.The polyamide resin was then desorbed with 60%ethanol with a desorption rate of up to 75.9%.

关 键 词：三叶青 总黄酮 聚酰胺树脂 纯化 

分 类 号：S567[农业科学—中草药栽培]