赤芝中腺苷和尿苷HPLC指纹图谱及含量分析  

HPLC fingerprint and content analysis of adenosine and uridine in Ganoderma lucidum

作　　者：林韧安 李静静[2] 林琳 黄爱云 管楚韵 章海英 张伊涵 林子琪 刘京晶[2] LIN Ren′an;LI Jingjing;LIN Lin;HUANG Aiyun;GUAN Chuyun;ZHANG Haiying;ZHANG Yihan;LIN Ziqi;LIU Jingjing(Yueqing City Natural Resources and Planning Bureau,Yueqing 325600,Zhejiang;State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou 311300,Zhejiang;Zhejiang Institute of Subtropical Crops,Wenzhou 325005,Zhejiang)

机构地区：[1]乐清市自然资源和规划局,浙江乐清325600 [2]浙江农林大学省部共建亚热带森林培育国家重点实验室,浙江杭州311300 [3]浙江省亚热带作物研究所,浙江温州325005

出　　处：《浙江农业科学》2024年第8期1764-1769,共6页Journal of Zhejiang Agricultural Sciences

摘　　要：采用指纹图谱对赤芝水溶性成分中的腺苷和尿苷进行检测及含量测定,寻找不同来源孢子粉和子实体的含量变化规律,结果表明,不同来源和处理的灵芝粉和孢子粉在指纹图谱上有明显差异。赤芝菌盖中尿苷含量为0.072~0.849 mg·g^(-1),菌柄中尿苷含量为0.056~0.302 mg·g^(-1),破壁孢子粉中尿苷含量为0.032~0.089 mg·g^(-1);赤芝菌盖中腺苷含量为0.019~0.118 mg·g^(-1),菌柄中腺苷含量为0.005~0.024 mg·g^(-1),破壁孢子粉中腺苷含量为0.024~0.107 mg·g^(-1);同品种不同潮次采收的未破壁孢子粉主要成分含量无较大差异;同一品种的破壁孢子粉和未破壁孢子粉之间也无明显差异,但同一品种不同批次的赤芝中腺苷和尿苷含量差异显著。因此,可利用高效液相色谱法指纹图谱在8.5~20.0 min的区域内破壁孢子粉只有一个峰鉴别出破壁孢子粉,利用尿苷和腺苷的含量鉴别孢子粉中是否含有灵芝粉。The fingerprint spectrum were used to detect and determine the content of adenosine and uridine in water-soluble components of Ganoderma lucidum,in order to find out the variation of the content of different spore powders and fruiting bodies.The results showed that there were significant differences in fingerprints between Ganoderma lucidum powder and spore powder from different sources;the content of uridine in the pileus of Ganoderma lucidum was 0.072-0.849 mg·g^(-1),the content of uridine in the stipe was 0.056-0.302 mg·g^(-1),and the content of uridine in the broken spore powder was 0.032-0.089 mg·g^(-1);the content of adenosine in the pileus of Ganoderma lucidum was 0.019-0.118 mg·g^(-1),the content of adenosine in the stipe was 0.005-0.024 mg·g^(-1),and the content of adenosine in the broken spore powder is 0.024-0.107 mg·g^(-1);There was no significant difference in the content of the main components of the unbroken spore powder harvested from the same variety but with different tides.There was no significant difference between the broken spore powder and the unbroken spore powder of the same variety.The difference of adenosine and uridine content in different batches of the same variety was obvious.The identification of broken spore powder can be achieved by using the fingerprint spectrum of high-performance liquid chromatography(HPLC)with only one peak in the area of 8.5-20.0 minutes for broken spore powder.The content of uridine and adenosine is used to identify whether the spore powder contains Ganoderma lucidum powder.

关 键 词：赤芝 高效液相色谱法指纹图谱 水溶性物质 腺苷 尿苷 

分 类 号：S567.3[农业科学—中草药栽培]