猪源ETEC/STEC杂合大肠埃希菌菌株的分离、毒力基因鉴定及耐药性分析  

Isolation,virulence gene identification,and antimicrobial resistance analysis of hybrid enterotoxigenic and Shiga toxin-producing Escherichia coli(ETEC/STEC)strain from pigs

作　　者：蒋利明 陈怡洁 袁秀芳[2] 李军星[2] JIANG Liming;CHEN Yijie;YUAN Xiufang;LI Junxing(Taizhou Animal Disease Prevention and Control Center,Taizhou 318050,Zhejiang;Institute of Animal Husbandry and Veterinary Medicine,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,Zhejiang;College of Life Science and Engineering,Foshan University,Foshan 528200,Guangdong)

机构地区：[1]台州市动物疫病预防控制中心,浙江台州318050 [2]浙江省农业科学院畜牧兽医研究所,浙江杭州310021 [3]佛山大学生命科学与工程学院,广东佛山528200

出　　处：《浙江农业科学》2024年第8期1939-1943,共5页Journal of Zhejiang Agricultural Sciences

基　　金：台州市农业科技项目;浙江省自然科学基金(LY21C180002);浙江省重点研发计划(2019C02052-3)。

摘　　要：仔猪断奶后腹泻(PWD)通常是由产肠毒素大肠埃希菌(ETEC)感染导致的疾病,该菌通常携带LT、STa及STb等肠毒素中的一种或多种。猪水肿病又被称为胃肠道水肿病,是由产志贺氏毒素大肠埃希菌(STEC)感染引发的一种急性致死性传染病,具有较高的死亡率,该菌通常携带Stx1及Stx2等毒素。文章诊断了一例仔猪腹泻与水肿病并发引起高死亡率的病例,分离鉴定了一株同时携带产肠毒素大肠埃希菌(ETEC)及产志贺氏毒素大肠埃希菌(STEC)毒力基因的杂合菌株。经鉴定,杂合菌株携带黏附素F18基因、肠毒素基因(STa、STb、LT)以及水肿病致病毒素基因(Stx2e)。药敏试验结果表明,该大肠埃希菌分离株对头孢噻肟、阿米卡星等5种药物敏感,对壮观霉素、环丙沙星等2种药物中度敏感,对恩诺沙星、链霉素等14种药物耐药。该分离菌株为产肠毒素型与产志贺氏毒素杂合型大肠埃希菌(ETEC/ESTEC),对多种抗菌药具有较强的耐药性。Post weaning diarrhea(PWD)in piglets is usually caused by infection with enterotoxigenic Escherichia coli(ETEC),which typically carries one or more of the enterotoxins LT,STa,and STb.Pigt edema disease(ED),also known as bowel edema or gut edema,is an acute and lethal infectious disease caused by infection with Shiga toxin-producing Escherichia coli(STEC),with a high mortality rate.This bacterium usually carries toxins such as Stx1 and Stx2.In this study,we diagnosed a case of PWD complicated by ED with high mortality in piglets,and isolated and identified a hybrid strain carrying both enterotoxigenic Escherichia coli(ETEC)and Shiga toxin-producing Escherichia coli(STEC)virulence genes.It shows that the hybrid strain carried the F18 adhesin gene,enterotoxin genes(STa,STb,LT),and edema disease causing toxin gene(Stx2e).The results of antimicrobial sensitivity test showed that the E.coli isolate was sensitive to five drugs,including cefotaxime and amikacin,moderately sensitive to two drugs,including spectinomycin and ciprofloxacin,and resistant to 14 drugs,including enrofloxacin and streptomycin.The isolate is a hybrid Escherichia coli(ETEC/STEC),and shows strong resistance to multiple antibiotics.

关 键 词：仔猪断奶后腹泻 猪水肿病 分离鉴定 毒力基因 

分 类 号：S852.61[农业科学—基础兽医学]