基于模式病毒评价商品化消毒剂对非洲猪瘟病毒的消杀效果  

Evaluation of inactivation effect of commercial disinfectant on African swine fever virus based on model virus

作　　者：倪征[1] 朱寅初 孙冰冰[2] 云涛[1] 陈柳[1] 徐辉[2] 叶伟成[1] 华炯钢[1] 张存[1] NI Zheng;ZHU Yinchu;SUN Bingbing;YUN Tao;CHEN Liu;XU Hui;YE Weicheng;HUA Jionggang;ZHANG Cun(Institute of Animal Husbandry and Veterinary Medicine,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,Zhejiang;Zhejiang Provincial Center for Animal Disease Control,Hangzhou 310018,Zhejiang)

机构地区：[1]浙江省农业科学院畜牧兽医研究所,浙江杭州310021 [2]浙江省动物疫病预防控制中心,浙江杭州310018

出　　处：《浙江农业科学》2024年第8期1944-1949,共6页Journal of Zhejiang Agricultural Sciences

基　　金：浙江省“三农六方”科技协作项目(2019SNLF018);浙江省重点研发计划(2021C02050-2)。

摘　　要：为了解常用商品化消毒剂对非洲猪瘟病毒(ASFV)的消杀效果,为其消毒剂的筛选提供参考。该研究利用伪狂犬病毒(pseudorabies virus,PRV)与ASFV病毒理化特征的相似性,以PRV作为替代标识毒株,建立了消毒剂细胞评价模型,于体外检测了3种市售消毒剂对生长在BHK-21细胞中PRV的灭活效果。分别从3类消毒剂的不同方面对消毒剂抗病毒作用的影响进行消杀效果的评估。研究显示,有效含量为0.125 g·L^(-1)的戊二醛癸甲溴铵溶液,以及有效含量为0.5 g·L^(-1)的过氧乙酸溶液和二氯异氰脲酸钠,在室温作用30 min,均可有效灭活PRV。其中,过氧乙酸综合评价最优;戊二醛癸甲溴铵性价比最高,但所需消杀时间较长,受低温影响比较严重。该研究可为养殖场防控ASFV的感染提供消毒剂筛选的理论依据,并对ASFV的消毒标准提供参考。In order to evaluate and prove the elimination effect of commercial disinfectants on African swine fever virus(ASFV),and to provide reference for the screening of disinfectants.In this study,the similarity of physicochemical characteristics of pseudorabies virus(PRV)and ASFV virus was used to establish a disinfectant cell evaluation model with PRVas an alternative marker strain.The inactivation effect of three commercially available disinfectants on PRV grown in HBK-21 cells was tested in vitro.The disinfecting effect of the three disinfectants was evaluated from several aspects on the antiviral effect of the three disinfectants.The results showed that glutaraldehyde methyl bromide solution with an effective content of 0.125 g·L^(-1),peracetic acid solution with an effective content of 0.5 g·L^(-1) and sodium dichloroisocyanurate could effectively inactivate PRV at room temperature for 30 min.Among them,peracetic acid comprehensive evaluation is the best;Glutaraldehyde decyl methyl bromide is the most cost-effective,but it requires a long time of elimination and is seriously affected by low temperature.This study can provide theoretical basis for disinfectant screening for the prevention and control of ASFV infection in livestock farms,and provide reference for the establishment of disinfection standards for ASFV.

关 键 词：消毒剂细胞评价模型 ASFV PRV 杀灭病毒效果 

分 类 号：S852[农业科学—基础兽医学]