固定化微生物技术在小秦岭金矿区农田土壤修复中的应用  

Application of immobilized microorganism technology on soil remediation of farmland in Xiaoqinling gold mining area

作　　者：李立军 仲惟[1,2] 李涛 LI Lijun;ZHONG Wei;LI Tao(College of Applied Engineering,Henan University of Science and Technology,Luoyang 472000,Henan;Sanmenxia Vocational and Technical College,Sanmenxia 472000,Henan)

机构地区：[1]河南科技大学应用工程学院,河南洛阳471000 [2]三门峡职业技术学院,河南三门峡472000

出　　处：《浙江农业科学》2024年第8期1950-1956,共7页Journal of Zhejiang Agricultural Sciences

基　　金：国家自然科学基金项目(32200089);河南省科技攻关项目(222102110198)。

摘　　要：为积极探索土壤污染防治新路径,开展固定化微生物技术在土壤原位生态修复方面的应用研究。选取豫西小秦岭金矿矿区及周边农田土壤进行重金属含量测定及分析评价,发现该地区土壤中Pb^(2+)元素污染风险较大。将侧孢芽孢杆菌、放线菌及酵母菌株进行固定化混合菌制备并开展土壤原位生物修复。发现经固定化混合菌处理后土壤脲酶活性显著高于CK。以Pb^(2+)钝化效率及去除率提高为目的,分别采用单因素试验及响应面法对影响土壤修复效果各因素进行分析,最终确定Pb^(2+)最佳去除条件为固定化混合菌投加量10.496%,处理温度30.784℃,pH值6.242,Pb^(2+)去除率理论值为72.754%。对此模型进行试验验证,结果与理论值接近,说明响应面分析可起到优化土壤原位修复试验参数的目的。In order to explore new methods of soil pollution prevention,the application of immobilized microorganism technology in soil ecological remediation was studied.The heavy metal contents in the soil of Xiaoqinling gold mining area and the surrounding farmland in Western Henan Province were determined and evaluated,it was found that the risk of Pb^(2+)element pollution in the soil in this area was high.The mixed bacteria of Bacillus laterosporus,actinomycetes and yeast were immobilized to carry out soil bioremediation in situ,the results showed that the urease activities of soil treated with immobilized bacteria were significantly higher than that of CK.In order to improve the passivation efficiency and removal rate of Pb^(2+),the single factor test and response surface methodology were used to analyze the factors affecting the soil remediation effect,the optimal removal conditions of Pb^(2+)were determined finally:the dosage of immobilized mixed bacteria was 10.496%,the treatment temperature was 30.784℃,the pH value was 6.242,and the theoretical removal rate of Pb^(2+)was 72.754%.The model was verified by experiment,the result of experiment was close to the theoretical value,indicating that response surface analysis can optimize the soil remediation experimental parameters.

关 键 词：固定化微生物技术 土壤原位修复 去除率 单因素试验 响应面分析 

分 类 号：S-3[农业科学]