抑制牛磺酸上调基因1通过上调脑源性神经生长因子减轻大鼠脑缺血再灌注损伤  

Inhibition of taurine upregulation gene 1 reduces cerebral ischemia⁃reperfusion injury in rats by upregulating brain⁃derived nerve growth factor

作　　者：李斐[1] 江洪祥 张惠凯 张新元 陈谦学[1] LI Fei;JIANG Hongxiang;ZHANG Huikai;ZHANG Xinyuan;CHEN Qianxue(Dept.of Neurosurgery,Renmin Hospital of Wuhan University,Wuhan 430060,Hubei,China)

机构地区：[1]武汉大学人民医院神经外科,湖北武汉430060

出　　处：《武汉大学学报（医学版）》2024年第8期898-902,共5页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:82071299)。

摘　　要：目的:探讨牛磺酸上调基因1(TUG1)在大鼠脑缺血再灌注损伤(CIRI)中的作用及其可能的机制。方法:建立CIRI大鼠模型,分别转染反义寡核苷酸(ASO)-TUG1及其空白对照ASO-NC,将大鼠分为对照组、CIRI组、CIRI+ASO-NC组、CIRI+ASO-TUG1组,采用mNSS评估各组大鼠的神经功能,TTC染色观察各组大鼠的脑梗死体积,PCR检测大鼠脑组织中TUG1和脑源性神经生长因子(BDNF) mRNA的表达水平,Western Blot和免疫组化实验检测各组BDNF蛋白的表达。结果:CIRI组大鼠脑组织中TUG1的表达水平显著高于对照组(P<0.05);转染ASO-TUG1可显著降低TUG1的表达水平(P<0.05);CIRI+ASO-TUG1组大鼠的mNSS评分和脑梗死体积显著低于CIRI组和CIRI+ASO-NC组(P<0.05);CIRI+ASO-TUG1组大鼠脑组织中BDNF mRNA和蛋白表达水平均高于CIRI组和CIRI+ASO-NC组(P<0.05)。结论:降低TUG1的表达则可显著增加CIRI大鼠脑组织中BDNF基因和蛋白的表达水平,减少脑梗死体积,改善神经功能。Objective To investigate the role of taurine upregulated gene 1(TUG1)in cerebral ischemia⁃reperfusion injury(CIRI)in rats and its possible mechanism.Methods A CIRI rat model was established and transfected with antisense oligonucleotides(ASO)⁃TUG1 and its blank control ASO⁃NC.All rats were divided into the control group,CIRI group,CIRI+ASO⁃NC group,and CIRI+ASO⁃TUG1 group.The modified neurological severity score(mNSS)was used to evaluate the neurological function of rats in each group.TTC staining was used to observe the volume of cerebral infarction in rats.The expression levels of TUG1 and brain⁃derived neurotrophic factor(BDNF)mRNA were detected by PCR.Western Blot and immunohistochemistry experiments were used to detect the expression of BDNF protein in each group.Results The expression level of TUG1 in the brain tissue of rats in the CIRI group was significantly higher than that in the control group(P<0.05).In addition,ASO⁃TUG1 significantly reduced the expression level of TUG1(P<0.05).The mNSS score and cerebral infarction volume of rats in the CIRI+ASO⁃TUG1 group were significantly lower than those in the CIRI group and CIRI+ASO⁃NC group(P<0.05).The expression levels of BDNF mRNA and protein in the brain tissue of rats in the CIRI+ASO⁃TUG1 group were higher than those in the CIRI group and the CIRI+ASO⁃NC group(P<0.05).Conclusion Downregulating the expression of TUG1 can significantly increase BDNF mRNA and protein expression levels in the brain tissue of CIRI rats,reduce the volume of cerebral infarction,and improve the rats'neurological function.

关 键 词：牛磺酸上调基因1 脑缺血再灌注损伤 脑源性神经生长因子 神经功能 

分 类 号：R651.1[医药卫生—外科学]