载多柔比星双介孔二氧化硅纳米球的表征及体外抗癌研究  

Characterization and in Vitro Anticancer Ability of Doxorubicin Loaded Bimodal Mesoporous Silica Nanospheres

作　　者：聂晓娅 张小娟[1] 张艳[1] 葛少兵 胡清伟[1,2] NIE Xiaoya;ZHANG Xiaojuan;ZHANG Yan;GE Shaobing;HU Qingwei(Chongqing Medical and Pharmaceutical College,Chongqing 401331,China;Chongqing Engineering Research Center of Pharmaceutical Sciences,Chongqing 401331,China;Northwestern Polytechnical University,Xi'an 710072,China)

机构地区：[1]重庆医药高等专科学校,重庆401331 [2]重庆市药物制剂工程技术研究中心,重庆401331 [3]西北工业大学,西安710072

出　　处：《中国药学杂志》2024年第14期1331-1338,共8页Chinese Pharmaceutical Journal

基　　金：重庆市自然科学基金面上项目资助(cstc2020jcyj-msxmX0627);重庆市教委科学技术研究青年项目资助(KJQN201902803);重庆医药高等专科学校科研教研项目资助(CQYGZJG2003)。

摘　　要：目的 制备载多柔比星(doxorubicin,DOX)的双介孔二氧化硅纳米球(bimodal mesoporous silica nanospheres,BMSNs),并评估其体外吸附/脱附特性和对肿瘤细胞增殖的抑制作用。方法 通过溶胶-凝胶法和溶剂热处理途径合成BMSNs;采用扫描电镜、比表面积及孔隙度分析仪、傅里叶变换红外光谱仪、紫外-可见光谱仪等方法对BMSNs进行分析和表征;采用间歇式吸附实验考察BMSNs对DOX的吸附/脱附特性;采用CCK8法检测载DOX双介孔二氧化硅纳米球BMSNs-DOX对人乳腺癌细胞MCF-7和人肺癌细胞A549增殖抑制作用。结果 BMSNs对DOX吸附/脱附能力明显优于典型均匀介孔二氧化硅MCM-41。在pH=7.0时,BMSNs对DOX的吸附量高达91.7μmol·g^(-1)(即载药量5.32%),是MCM-41吸附量的1.6倍。在pH=5.0缓冲溶液中,BMSNs-DOX对DOX分子的脱附(释放)效率可达约96%,脱附时间仅需约30 min。与MCM-41-DOX和DOX相比,BMSNs-DOX对人乳腺癌细胞MCF-7和人肺癌细胞A549增殖抑制作用更强,在4μg·mL^(-1)质量浓度时,癌细胞存活率仅为23.67%和15.79%。结论 相比于MCM-41,BMSNs能更快、更多地对癌症细胞释放抗癌药物DOX,BMSNs-DOX对MCF-7细胞和A549细胞有较强的杀伤作用,是一种具有潜在应用价值的新型给药系统。OBJECTIVE To prepare bimodal mesoporous silica nanospheres loaded with doxorubicin,and evaluate their adsorption/desorption properties and proliferation inhibition effects on cancer cells.METHODS bimodal mesoporous silica nanospheres(BMSNs) were prepared using sol-gel method and thermal treatment.The nanospheres were analyzed and characterized using scanning electron microscopy,specific surface area and porosity analyzer,Fourier transform infrared spectroscopy,and UV-VIS spectrometer.The adsorption/desorption characteristics of DOX by BMSNs were investigated by adsorption experiment.The proliferation inhibition effects of BMSNs-DOX on MCF-7 cells and A549 cells were detected using CCK8 method.RESULTS Compared with mesoporous MCM-41,which has typical uniform mesopores,BMSNs could provide more favorable matter diffusion and adsorption capacity towards DOX due to their more expansive pore structure.At pH=7.0,BMSNs exhibited a high adsorption capacity for DOX of 91.7 μmol·g~(-1)(equivalent to a drug loading capacity of 5.32%),which was 1.6 times higher than that of MCM-41.In pH=5.0 buffer solution,BMSNs-DOX desorbed(released) DOX molecules with an efficiency of about 96%,in just 30 minutes.BMSNs-DOX exhibited a stronger inhibitory effect than MCM-41-DOX and DOX.At a concentration of 4 μg·mL~(-1),the survival rate of MCF-7 cells and A549 cells was only 23.67% and 15.79% respectively.CONCLUSION Compared with MCM-41,BMSNs is expected to release DOX to cancer cells more efficiently.BMSNs-DOX exhibits a significant inhibitory effect on MCF-7 cells and A549 cells,suggesting its potential as a drug carrier to enhance the cytotoxicity of chemotherapy drugs on cancer cells.

关 键 词：双介孔二氧化硅纳米球 多柔比星 吸附 脱附 抗癌 

分 类 号：R944[医药卫生—药剂学]