抗菌涂料抗菌性能测定方法的扩展与分析  

Antibacterial Determining and Properties of Antibacterial Coatings Aganist Four Bacteria

作　　者：赵培 王伟科 胡家应 杨然存 徐鹏 ZHAO Pei;WANG Weike;HU Jiaying;YANG Rancun;XU Peng(Coating Center,Henan Institute of Product Quality Inspection Technology,Zhengzhou 450047,China)

机构地区：[1]河南省产品质量检验技术研究院涂料中心,郑州450047

出　　处：《河南科学》2024年第8期1138-1144,共7页Henan Science

基　　金：河南省科技攻关项目(242102411010)。

摘　　要：为更好分析不同抗菌涂料对多种细菌的抗菌效果,先利用梯度稀释法确定四种细菌菌液的适宜测试浓度,消除菌液丰度的影响,然后按《抗菌涂料(漆膜)抗菌性测定法和抗菌效果》(GB/T 21866—2008)与《食品卫生微生物学检验菌落总数测定》(GB/T 4789.2—2016)等标准方法,测定了硅酸盐和竹炭两种类型抗菌涂料对大肠杆菌、金黄色葡萄球菌、肺炎克雷伯氏菌、铜绿假单胞菌的抗菌性能及其耐久性.结果表明,菌悬液制备过程中菌落的适宜计数稀释梯度均为10^(-5)和10^(-6);在24 h后两种抗菌涂料对4种细菌的抗菌率和抗菌耐久性分别达到99.35%和98.18%以上,对比非抗菌涂料,抗菌性能达到Ⅰ级标准.两种抗菌涂料均适用于医院、幼儿园等公共卫生条件要求较高的场所.To compare the antibacterial effects of different antibacterial coatings,the antibacterial properties and durability of the silicate and bamboo charcoal antibacterial coatings to four bacteria,including Escherichia coli,Staphylococcus aureus,Klebsiella pneumoniae and Pseudomonas aeruginosa,were determined according to the standards of Test Method and Effect for Antibacterial Capability of Paints Film(GB/T 21866-2008)and Microbiological Testing of Food:Determination of Total Bacterial Count(GB/T 4789.2-2016).The suitable counting dilution gradients of colonies in the preparation of bacterial suspension are 10^(-5)and 10^(-6).The antibacterial rates and durability of the two antibacterial coatings to four bacteria reach above 99.35% and 98.18% after 24 h,respectively,and both antibacterial performance standards reach to grade Ⅰ.The two coatings are suitable for hospitals,kindergartens and other places where require higher public health conditions.

关 键 词：抗菌涂料 菌悬液 抗菌性能 大肠杆菌 金黄色葡萄球菌 肺炎克雷伯氏菌 铜绿假单胞菌 

分 类 号：TQ630.7[化学工程—精细化工]