石墨炉原子吸收标准加入校正法测定血中铅  

Determination of lead in blood by graphite furnace atomic absorption standard addition correction method

作　　者：陶红霞[1] 卢俊霖[1] 张家树[1] 潘泓宇[1] 钱炳富 邓航 张大芬[1] 谢若愚 TAO Hongxia;LU Junlin;ZHANG Jiashu;PAN Hongyu;QIAN Bingfu;DENG Hang;ZHANG Dafen;XIE Ruoyu(Panzhihua Center for Disease Control and Prevention,Panzhihua 617000,Sichuan Province,China)

机构地区：[1]攀枝花市疾病预防控制中心,四川攀枝花617000

出　　处：《预防医学情报杂志》2024年第8期1031-1034,1042,共5页Journal of Preventive Medicine Information

基　　金：攀枝花市科技计划项目(项目编号:2023ZD-S-39)。

摘　　要：目的采用石墨炉原子吸收标准加入校正法即优化原子吸收石墨炉测定血液中铅的方法,为改进检测方法提供参考。方法血液通过一定比例的硝酸、曲拉通稀释,用1%磷酸二氢铵作基体改进剂,牛血做本底,使用标准加入校正法测定标准曲线后再进行样品的定量测定。所得数据采用Excel 2019软件进行配对t检验,检验水准为0.05。结果本方法对血铅的测定在0~500μg/L范围里吸光度和铅含量成正比、线性良好相关系数R^(2)=0.998检出限0.67μg/L、精密度0.8%~3.15%、回收率97%~104%都达到要求。结论本法全面优化血中铅的测定条件,简单、高效准确,为血中铅测定较为理想的测定方法,可用于普通人群和接触人群中血铅的检测与评价。Objective To optimize the determination of lead in blood by graphite furnace atomic absorption standard addition correction method,and to provide reference for improving the detection method.Methods Blood was diluted with a certain proportion of nitric acid and triton,and 1%ammonium dihydrogen phosphate was used as the base modifier and bovine blood was used as the background.The standard curve was determined by the standard addition correction method,and then the sample was quantitatively determined.The obtained data were examined by paired t-test by Excel 2019.And the test level wasα=0.05.Results In the range of 0-50 ug/L,the absorbance of blood lead was proportional to the lead content by this method.With a linear good correlation coefficient of R^(2)=0.998,the detection limit was 0.67 ug/L,the precision was 0.8%-3.15%,and the recovery rate was 97%-104%,all meeting the requirements.Conclusions This method potimized the conditions for the determination of lead in blood,is simple,efficient and accurate,and is an ideal method for the determination of lead in blood.It can be used for the detection and evaluation of lead in blood in general population and contact population.

关 键 词：血铅 标准加入校正法 自动进样 原子吸收 

分 类 号：R446.1[医药卫生—诊断学] O657.31[医药卫生—临床医学]