河曲丝叶蜂对寄主白柳部分挥发物的触角电位和行为反应  

Electroantennogram and behavioral responses of Nematus hequensis to a part of host volatiles about Salix alba

作　　者：唐晓琴 宋振浩 孙兆旭 郭鑫楠 董晓 臧建成 俞焜 赵远 TANG Xiaoqin;SONG Zhenhao;SUN Zhaoxu;GUO Xinnan;DONG Xiao;ZANG Jiancheng;YU Kun;ZHAO Yuan(Plant Sciences College,Xizang Agricultural&Animal Husbandry University;Lab of Resource and Applied Insects in the Xizang Plateau;Key Laboratory of Forest Ecology in the Xizang Plateau(Xizang Agricultural&Animal Husbandry University),Ministry of Education,Linzhi 860000,Xizang,China;Institute of Agricultural Research,Xizang Autonomous Region Academy of Agricultural and Animal Husbandry Sciences,Lasa 850000,Xizang,China)

机构地区：[1]西藏农牧学院植物科学学院 [2]西藏高原资源昆虫与应用昆虫实验室 [3]西藏高原森林生态教育部重点实验室,西藏林芝860000 [4]西藏自治区农牧科学院农业研究所,西藏拉萨850000

出　　处：《高原农业》2024年第4期357-366,共10页Journal of Plateau Agriculture

基　　金：西藏自治区科技计划项目(XZ202201ZY0015N、XZ202301ZY0019N);西藏农牧学院研究生教育创新计划项目(YJS2023-44)。

摘　　要：根据河曲丝叶蜂(Nematus hequensis)对不同寄主挥发物的触角电位和行为反应,以期筛选出对该害虫成虫具有较强引诱作用的白柳植物挥发物和其最佳浓度,为该害虫的无害防治提供理论依据。在河曲丝叶蜂成虫羽化高峰期,使用固相微萃取和气相色谱质谱联用仪(GC-MS)鉴定白柳(Salix alba L.)挥发物成分;以正己烷为对照,利用触角电位仪(electroantennogram,EAG)测定河曲丝叶蜂1日龄未交配雌雄成虫对5种寄主植物挥发物的EAG反应,使用Y型嗅觉仪测试其对5种寄主挥发物不同浓度的行为反应。EAG反应结果表明:河曲丝叶蜂雌、雄成虫对于5种化合物均有较明显的电生理反应,雄虫在叶醇为1 000 ng·μL^(-1)和10 000 ng·μL^(-1)时引起较显著的EAG反应,并与其余4种化合物之间差异明显;雌虫在叶醇、丁香酚为1 000 ng·μL^(-1)时引起较明显的EAG反应,并与其余3种化合物之间差异明显。Y型嗅觉仪生测结果表明:只有丁香酚在1 000 ng·μL^(-1)、10 000 ng·μL^(-1)时对河曲丝叶蜂雌、雄成虫的引诱率与对照相比差异性显著(P<0.05)或极显著(P<0.01)。白柳挥发物中的丁香酚对河曲丝叶蜂未交配雌雄成虫有较好的引诱活性,说明其在河曲丝叶蜂识别寄主过程中有重要作用,可以用于开发该害虫的植物源引诱剂。Based on the electroantennogram(EAG)and behavioral responses of Nematus hequensis to different host volatiles,The aim was to screen out the Salix alba volatiles with strong attractiveness on the adult of this pest and the optimal concentration,so as to provide a theoretical basis for the harmless control of this pest.the volatiles of S.alba were identified by solid-phase microextraction(SPME)and gas chromatography-mass spectrometry(GC-MS)at the peak of adult plumage.N-hexane was used as the control,the 1 day-old unmated females and males were determined by EAG to the five volatiles of S.alba;and the 1 day-old unmated females and males were determined by Y-tube olfactometer to different concentration gradients volatiles of five host plants.The results showed that unmated adult females and males of the N.hequensis showed obvious electrophysiological responses to the five compounds.Male adults showed significant EAG responses at 1000 ng·μL^(-1) and 10000 ng·μL^(-1) of leaf alcohol,which were significantly different from the other four compounds.When the concentration of leaf alcohol and eugenol was 1000 ng·μL^(-1),the female caused obvious EAG response,which was significantly different from the other three compounds.The results of the Y-tube olfactometer bioassay showed that only eugenol at 1000 ng·μL^(-1) and 10000 ng·μL^(-1) had significant or extremely significant differences in the attraction rate of male and female adults of N.hequensis compared with the control.The eugenol in the volatiles of S.alba had good attractive activity to the unmated male and female adults of N.hequensis,indicating that it played an important role in the host identification process of N.hequensis and could be used to develop plant-derived attractants for the pest.

关 键 词：河曲丝叶蜂 寄主挥发物 触角电位 行为反应 

分 类 号：Q966[生物学—昆虫学]