天山堇菜提取物对甲型H1N1流感病毒感染小鼠的抗病毒及免疫调节作用研究  

Study on the Antiviral and Immunomodulatory Effects of Viola Tianshanica Maxim Extract on Mice Infected with Influenza A H1N1 Virus

作　　者：包蕾[1] 刘燕 耿子涵 王雪 BAO Lei;LIU Yan;GENG Zihan;WANG Xue(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Xinjiang Institute of Materia Medica,Urumqi 830000,China)

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]新疆维吾尔自治区药物研究所,乌鲁木齐830000

出　　处：《病毒学报》2024年第4期695-704,共10页Chinese Journal of Virology

基　　金：新疆维吾尔自治区自然科学基金杰出青年科学基金:(项目号:2021D01E26),题目:基于Toll样受体3/7(TRL3/7)、维甲酸诱导基因1(RIG⁃1)受体探讨天山革菜总黄酮对流感病毒感染引起宿主细胞固有免疫应答的调控机制;自治区创新环境(人才、基地)建设专项:(项目号:PT2305),题目:地产中药民族药活性筛选、药效评价及机制研究平台建设。

摘　　要：明确天山堇菜提取物的抗甲型H1N1流感病毒药效,探索其免疫调节的作用机制。采取甲型H1N1感染小鼠致肺炎模型,分为正常组、模型组、达菲组、天山堇菜提取物320、160、80 mg·kg-1剂量组,检测肺指数、肺组织病毒载量、肺组织中免疫因子、脾组织中免疫细胞Th1/Th2、Th17/Treg比例、脾组织中NK细胞百分比和激活量;进行肺部病理学观察。与正常组比较,模型组小鼠肺指数显著升高(P<0.01);病毒载量显著上升(P<0.01);TNF-α、TGF-β含量显著升高(P<0.01),IL-17A、IFN-γ有升高趋势但无统计学差异,IL-12基本无差别;Th1/Th2比例显著升高(P<0.05),Th17/Treg有降低趋势,但无显著性差异;脾组织中总NK细胞百分比显著升高(P<0.01)、活化的NK细胞占比显著升高(P<0.05);肺组织间质出现大范围的炎症渗出,伴随明显的瘀血现象,且肺组织间质有粘液渗出。与模型组比较,天山堇菜提取物能够显著降低小鼠肺指数(P<0.05);降低小鼠肺组织中病毒载量(P<0.01);降低TNF-α和TGF-β含量,升高IL-17A、IFN-γ和IL-12含量(P<0.01);增加活化NK细胞数量(P<0.01);并能够改善肺组织病变情况。天山堇菜提取物能够通过减轻肺部损伤、降低病毒载量、抑制炎症反应、调节关键细胞因子以及激活免疫细胞等多重机制发挥抗甲型H1N1流感病毒感染的作用。To clarify the anti-influenza A(H1N1)effect of Viola tianshanica Maxim extract and explore its immunomodulatory mechanism.The mouse pneumonia model induced d by H1N1 infection was divided into normal group,model group,Tamiflu group,and Viola tianshanica Maxim extract 320,160,and 80 mg·kg-1 dose groups.The lung index,viral load in lung tissue,immune factors in lung tissue,immune cell Th1/Th2,Th17/Treg ratio in spleen tissue,total amount and activation of NK cells in spleen tissue were detected;lung pathologic change was observed.Compared with the normal group,the lung index of mice in the model group was significantly increased(P<0.01);the viral load was significantly increased(P<0.01);the contents of TNF-αand TGF-βwere significantly increased(P<0.01).,IL-17A and IFN-γtended to increase but there was no statistical difference.IL-12 basically showed no difference.The ratio of Th1/Th2 increased significantly(P<0.05),and Th17/Treg showed a decreasing trend but no significant difference.The total NK cell amount in the spleen tissue increased significantly(P<0.01),and the activated NK cell content increased significantly(P<0.05).There was a large-scale inflammatory infiltration in the interstitium of the lung tissue,accompanied by obvious stasis.There is bleeding phenomenon,and there is mucus exudation in the interstitium of the lung tissue.Compared with the model group,Viola tianshanica Maxim extract can significantly reduce the lung index of mice(P<0.05);reduce the viral load in mouse lung tissue(P<0.01);reduce the contents of TNF-αand TGF-β,and increase IL-17A,IFN-γand IL-12 contents(P<0.01).It can increase the number of activated NK cells(P<0.01),and improve lung tissue lesions.Viola tianshanica Maxim extract exerts anti-influenza A(H1N1)virus infection through multiple mechanisms such as reducing lung damage,reducing viral load,inhibiting inflammatory response,regulating key cytokines,and activating immune cells.

关 键 词：天山堇菜提取物 甲型H1N1流感病毒 肺炎 免疫调节 抗病毒 

分 类 号：R373.1[医药卫生—病原生物学]