基于生物信息学分析铁死亡与猴痘感染的相关性  

Bioinformatics Analysis⁃based Investigation of the Connection Between Mpox Infection and Ferroptosis

作　　者：潘静怡 詹晨昕 张安琪[1] PAN Jingyi;ZHAN Chenxin;ZHANG Anqi(Department of Anesthesiology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325000,China;Department of Cardiovascular Medicine,Wenzhou Central Hospital,Wenzhou 325000,China)

机构地区：[1]温州医科大学附属第一医院麻醉科,温州325000 [2]温州市中心医院心血管内科,温州325000

出　　处：《病毒学报》2024年第4期757-771,共15页Chinese Journal of Virology

基　　金：温州市级科技计划项目(项目号:Y2023568),题目:基于生物信息学预测脓毒症肺损伤双硫死亡靶点;浙江省医药卫生科技计划项目(项目号:2023RC045),题目:二氯乙酸钠靶向NLRP3炎症小体缓解急性肺损伤的作用及机制研究。

摘　　要：猴痘是一种由猴痘病毒引起的传染病。铁死亡是一种铁依赖性的细胞死亡方式。目前,很少有关于猴痘病毒感染过程是否有铁死亡相关机制参与的研究。本研究探索了猴痘病毒感染与铁死亡之间可能的分子机制和生物学联系。本文在GEO(Gene expression omnibus)数据库中选择编号GSE(Gene expression omnibus series)36854数据集来分析并鉴定了540个猴痘相关差异表达基因。在FerrDb网站收集铁死亡相关基因后,利用维恩图鉴定猴痘差异表达基因与铁死亡基因之间的13个共有基因。对共有基因进行基因本体(Gene ontology,GO)与京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)富集分析后发现,共有基因主要富集在对脂多糖反应、细胞质核糖核蛋白颗粒、细胞因子活性等生物学功能,主要通过卡波西肉瘤相关疱疹病毒感染、IL17信号通路等通路参与猴痘疾病进程。利用NetworkAnalyst平台分别构建了TF(Transcription factor)⁃共有基因、共有基因⁃miRNA(microRNA,miRNA)、TF⁃共有基因⁃miRNA、共有基因相关蛋白质⁃化学物的相关互作调控网络,并探讨了共有基因与不同互作网络的密切联系。这些研究有助于探索铁死亡与猴痘病毒感染间的潜在分子机制和生物学联系,为猴痘的防治提供新的思路和治疗靶点。Mpox(known formerly as“monkeypox”)is an infectious disease caused by the mpox virus.Ferroptosis is an iron⁃dependent form of cell death.Few studies have focused on whether the mechanism of ferroptosis is involved in infection by the mpox virus.We explored the possible molecular mechanisms and biological links between mpox and ferroptosis.The Gene Expression Omnibus Series 36854 dataset from the Gene Expression Omnibus database was selected to identify and analyze 540 mpox⁃related differentially expressed genes(DEGs).After collecting ferroptosis⁃related genes on the FerrDb website,Venn diagram was used to identify 13 common genes between the DEGs of mpox and ferroptosis⁃related genes.The Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases revealed that common genes were mainly concentrated in biological functions such as“response to lipopolysaccharide”,“cytoplasmic ribonucleoprotein granule”,“cytokine activity”,and were mainly involved in the process of mpox through“Kaposi sarcoma⁃associated herpesvirus infection”and the“IL17 signaling pathway”.By using the NetworkAnalyst platform,transcription factor(TF)⁃common gene,common gene–microRNA,TF–common gene–microRNA,and common gene⁃related protein–chemicals correlated regulatory networks were constructed,respectively.The close relationship between common genes and different interaction networks was explored.Our results could help exploration of the potential molecular mechanisms and biological links between ferroptosis and infection by the mpox virus,and provide new ideas and therapeutic targets for the prevention and treatment of mpox.

关 键 词：猴痘 铁死亡 生物信息学 共有基因 网络分析 

分 类 号：R373.3[医药卫生—病原生物学]