核酸联检反应性鉴别非反应性献血者中隐匿性乙肝感染状况分析  

Analysis of Occult Hepatitis⁃B Infection in Non⁃discriminating Reactive Donors Based on the Nucleic⁃acid Test

作　　者：朱守兵 陆盈[2] 孙建杨 钦博 ZHU Shoubing;LU Ying;SUN Jianyang;QIN Bo(The Blood Center of Shaoxing,Shaoxing 312000,China;The Blood Center of Zhejiang,Hangzhou 310052,China;Shaoxing Maternal and Child Health Care Hospital,Shaoxing 312000,China)

机构地区：[1]绍兴市中心血站,绍兴312000 [2]浙江省血液中心,杭州310052 [3]绍兴市妇幼保健院,绍兴312000

出　　处：《病毒学报》2024年第4期837-842,共6页Chinese Journal of Virology

基　　金：浙江省基础公益研究计划项目(项目号:LGF22H190009),题目:基于碱基编辑系统失活HBV关键基因的抗病毒研究。

摘　　要：研究联检反应性鉴别非反应性献血者(Non⁃discriminating reactive donation,NDRD)中隐匿性乙肝感染(Occult hepatitis⁃B virus infection,OBI)情况,对既往在绍兴地区献血的NDRD进行追踪,首先使用化学发光试验检测HBsAg,使用核酸检测技术检测HBV DNA,首次追踪检测增加化学发光试验检测HBsAb,同时选取HBsAg阴性且HBV DNA也阴性合格献血者标本76份和确认为OBI的献血者标本52份作为对照检测HBsAb,经追踪检测HBsAg阴性且HBV DNA阳性的献血者判定为OBI,对不同人群血清中HBsAb阳性比例进行统计分析,对不同性别、不同年龄、不同学历、不同血清HBsAb浓度及不同追踪检测次数确定为OBI的情况进行统计分析。本次研究共追踪检测66人,其中经确认为OBI的12人,NDRD中OBI的比例18.18%,不同性别、不同学历的献血者确认为OBI的比例差异无统计学意义(P>0.05),不同年龄组的献血者确认为OBI的比例差异有统计学意义(P<0.05),HBsAg阴性且HBV DNA阴性合格献血者、NDRD和确认为OBI的献血者中HBsAb阳性的比例差异无统计学意义(P>0.05),NDRD中不同血清HBsAb浓度的献血者确认为OBI的比例差异有统计学意义(P<0.05),不同追踪检测次数组OBI检出比例有统计学差异(P<0.05)。NDRD中OBI的比例较高,年龄越大的NDRD确定为OBI比例越高,血清HBsAb浓度大于300IU/L的NDRD中未发现OBI,HBsAb浓度大于300IU/L可以用作NDRD归队和保留的指标,追踪检测5次及以上的NDRD确认为OBI的比例明显增高,为增加NDRD中OBI的检出率,可以增加核酸检测次数至5次及以上。We wished to explore occult hepatitis⁃B infection(OBI)in non⁃discriminating reactive donors(NDRDs)based on the nucleic⁃acid test in Shaoxing,China.A chemoluminescence test was used to detect hepatitis B surface antigen(HBsAg).The nucleic⁃acid test was used to detect hepatitis⁃B virus(HBV)DNA.A chemiluminescence test was employed to detect hepatitis B surface antibody(HBsAb)for the first tracking test.Simultaneously,76 samples of blood from donors negative for HBsAg and HBV DNA were selected.Fifty⁃two samples of blood from donors confirmed as having OBI were selected as the control to detect HBsAb.Blood donors negative for HBsAg and positive for HBV DNA were determined to have OBI.Statistical analyses were conducted on the proportion of HBsAb that was positive in different populations.Statistical analyses were conducted on NDRDs confirmed as having OBI based on sex,age,educational background,HBsAb concentration in serum,and frequency of the tracking test.Sixty⁃six individuals were tracked and tested,of which 12 were confirmed to have OBI.The proportion of OBI in NDRDs was 18.18%.There was no significant difference in the proportion of blood donors confirmed as having OBI according to sex and educational background(P>0.05).There was a significant difference in the proportion of blood donors confirmed as having OBI in different ages(P<0.05).There was no significant difference in the proportion of HBsAb that was positive among blood donors who were negative for HBsAg and HBV DNA,NDRDs,and those confirmed as having OBI(P>0.05).There was a significant difference in the proportion of blood donors confirmed as having OBI in different HBsAb concentrations in serum in NDRDs(P<0.05).There was a significant difference in the proportion of OBI detection in different tracking tests(P<0.05).The proportion of OBI in NDRDs was high.The older the NDRD,the higher the proportion of OBI.OBI was not found in NDRDs with a HBsAb concentration in serum>300 IU/L.A HBsAb concentration in serum>300 IU/L could be used as an ind

关 键 词：核酸联检反应性鉴别非反应性 献血者 核酸检测 隐匿性乙肝感染 HBsAb 检测次数 

分 类 号：R512.6[医药卫生—内科学]