免疫芯片检测技术应用研究进展  

Research Progress on Immune Chips Detection Technology

作　　者：向钰洁 马学军[1,2] 申辛欣[1,2] XIANG Yujie;MA Xuejun;SHEN Xinxin(National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;National Key Laboratory for Early Warning and Intelligent Decision-making of Infectious Disease Tracing.Key Laboratory of Medical Viruses and Viral Diseases,National Health Commission.National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京102206 [2]传染病溯源预警与智能决策全国重点实验室.国家卫生健康委员会医学病毒和病毒病重点实验室.中国疾病预防控制中心病毒病预防控制所,北京102206

出　　处：《病毒学报》2024年第4期869-876,共8页Chinese Journal of Virology

基　　金：国家自然科学基金(项目号:82202593),题目:重组酶介导的核酸等温扩增(RAA)检测体系的优化及其在新型冠状病毒和流感病毒A/B型检测中的应用;国家重点研发计划(项目号:2021YFC2301102),题目:多技术融合的病原体免疫核酸双靶标快速超敏检测技术研究。

摘　　要：免疫芯片(Immune chips)是一种特殊的蛋白质芯片,该技术本质上是将抗原-抗体结合反应的特异性与电子芯片高密度集成的原理相结合,形成了一种新型的生物检测技术,具有高灵敏度、操作简便、反应速度快等优势。随着科学技术的进步和创新,免疫芯片技术正在不断突破原有的局限,向着更高的灵敏度、更宽的检测范围、更快的检测速度和更低的成本方向发展,成为检测领域强有力的工具。本文综述了几类免疫芯片的基本原理和技术特点,主要分析了其在病毒、细菌、疾病标志物、真菌、寄生虫等检测中的应用。Immune chips are special protein chips that essentially combine the specificity of antigen-antibody binding reactions with the high-density integration principle of electronic chips,forming a new type of biological detection technology with advantages such as high sensitivity,easy operation and fast response.This article reviews the basic principles and technical characteristics of several types of immune chips as well as their application progress in molecular detection of diseases,pathogens,agriculture and food safety.With the advancement and innovation of science and technology,the limitations of immune chip technology are continuously being broken overcome.The technology is evolving towards higher sensitivity,a wider detection range,faster detection speed,and lower cost,becoming a powerful tool in the field of molecular testing.

关 键 词：免疫芯片 微流控 液相芯片 非核酸物质 即时检测 

分 类 号：R373.9[医药卫生—病原生物学]