大鼠纳米氧化铟锡亚慢性暴露后体内的毒性效应  

Toxicity effects of indium tin oxide nanoparticles in rats after subchronic exposure

作　　者：刘楠 林殷乔 赵燕姿 陈晓阳 李小然 李纬康 曲晓雨 关毅 薛玲 LIU Nan;LIN Yinqiao;ZHAO Yanzi;CHEN Xiaoyang;LI Xiaoran;LI Weikang;QU Xiaoyu;GUAN Yi;XUE Ling(School of Public Health,North China University of Science and Technology,Hebei Key Laboratory of Occupational Health and Safety for Coal Industry,Tangshan 063210,China)

机构地区：[1]华北理工大学公共卫生学院,河北省煤矿卫生与安全重点实验室,河北省唐山市063210

出　　处：《中国煤炭工业医学杂志》2024年第3期232-237,共6页Chinese Journal of Coal Industry Medicine

基　　金：华北理工大学大学生创新创业计划(编号:X2022278,编号:X2022312)。

摘　　要：目的探讨纳米氧化铟锡(ITO-NPs)气管灌注12周亚慢性暴露后在大鼠体内的分布情况及各脏器的毒效应。方法将40只雄性Sprague-Dawley大鼠随机分为对照组(生理盐水)、低剂量组(1.2mg/kg·bw)、中剂量组(3mg/kg·bw)和高剂量组(6mg/kg·bw),每组10只。非暴露式气管灌注ITO-NPs,每周2次,间隔3天,连续染毒12周。染毒结束后处死动物,收集组织样品,包括脑、肺脏、肝脏、脾脏、肾脏、睾丸,ICP-MS法测定样品中铟元素含量,并对组织样品进行病理学观察。结果各剂量ITO-NPs暴露组中,大鼠相对肺重量均显著增加(P<0.05)。脑、肺脏、肝脏、脾脏、肾脏、睾丸的铟含量均呈剂量依赖性增加。1.2mg/kg·bw ITO-NPs组的TBIL(1.11±0.10)和BUN(4.92±0.36)水平显著高于对照组(P<0.05);3mg/kg·bw ITO-NPs组大鼠血清TBIL(1.06±0.16)、BUN(6.35±0.25)和MDA(7.59±0.72)含量明显高于对照组(P<0.05);6mg/kg·bw ITO-NPs组AST(183.77±29.18)、TBIL(1.06±0.13)、DBIL(0.54±0.05)、BUN(8.11±0.48)、UA(190.44±38.37)、Cr(245.54±49.21)和MDA(7.78±0.70)显著高于对照组(P<0.05)。在组织病理学上,检测到轻度至重度肺部炎症反应病灶以及急性炎症和肺泡蛋白沉积,这些病变的严重程度以剂量依赖的方式恶化。结论ITO-NPs暴露12周后,铟元素主要聚集肺组织中,其次是脾、肝、肾、睾丸和脑组织,且中、高剂量暴露可引起严重肺损伤。Objective To study the distribution the toxicity effects of indium tin oxide nanoparticles(ITONPs)in rats after 12w intratracheal instillation exposure.Methods A total of fourty male SD rats were randomly divided into four groups:control(physiological saline),low dose(1.2mg/kg·bw),medium dose(3mg/kg·bw),and high dose(6mg/kg·bw),with 10 rats in each group.Rats were intratracheally instilled ITO-NPs in 1ml using a syringe,twice a week,and sacrificed at week 12 post-exposure.After poisoning,animals were sacrificed and tissue samples were collected,including brain,lungs,liver,spleen,kidneys,and testes.The indium content in the samples was measured by ICP-MS,and the tissue samples were subjected to pathological observation.Results Relative lung weights were significantly increased in all ITONPs-exposed groups.All organs exhibited a statistically significant difference in indium levels.The TBIL(1.11±0.10)and BUN(4.92±0.36)levels in the 1.2mg/kg·bw ITO-NPs group were significantly higher than those in the control group(P<0.05);The serum TBIL(1.06±0.16),BUN(6.35±0.25),and MDA(7.59±0.72)levels in the 3mg/kg·bw ITO-NPs group rats were significantly higher than those in the control group(P<0.05);The AST(183.77±29.18),TBIL(1.06±0.13),DBIL(0.54±0.05),BUN(8.11±0.48),UA(190.44±38.37),Cr(245.54±49.21),and MDA(7.78±0.70).in the 6mg/kg·bw ITO-NPs group were significantly higher than those in the control group(P<0.05).Histopathologically,foci of slight-to-severe acute inflammatory response along with alveolar proteinosis were detected,and the severity of these lesions worsened in a dose-dependent manner.Conclusion After 12 weeks of exposure to ITO-NPs,indium mainly accumulates in lung tissue,followed by spleen,liver,kidney,testes,and brain tissue,and moderate to high doses of exposure can cause severe lung injury.

关 键 词：纳米氧化铟锡 亚慢性暴露 组织分布 病理学 

分 类 号：R99[医药卫生—毒理学]