黑龙江省稻田稗草对氰氟草酯抗性研究及药剂筛选  

Resistance of paddy Echinochloa crus-galli to cyhalofop-butyl and herbicide screening in Heilongjiang Province

作　　者：李广雨 黄元炬[1] 王春[1] 张苏新 王芊[1] LI Guangyu;HUANG Yuanju;WANG Chun;ZHANG Suxin;WANG Qian(Institute of Plant Protection,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China)

机构地区：[1]黑龙江省农业科学院植物保护研究所,哈尔滨150086

出　　处：《农药》2024年第8期620-624,共5页Agrochemicals

基　　金：“十四五”国家重点研发计划项目(2023YFD1401100);黑龙江省农业科技创新跨越工程重点攻关项目(CX23 GG14);黑龙江省农科院植物保护研究所青年基金项目(zbsqn2023-5)。

摘　　要：[目的]为明确黑龙江地区稻田稗草对氰氟草酯抗性水平,并筛选出轮换药剂。[方法]采用单剂量筛选法、整株生物测定法研究了33个供试稗草种群对氰氟草酯的抗性水平及对其他常用除草剂的交互及多抗性。[结果]单剂量筛选供试33个种群,除采自荒地的HL33以外,其余32个种群的相对鲜质量均大于25%,可以推测为疑似抗性种群。以HL33为敏感型计算抗性指数,9个供试种群:3个种群RI>10,表现为高水平抗性,占比33%;4个种群RI在5.28~9.96之间,表现为中水平抗性,占比44%;2个种群RI<5,表现为低水平抗性,发生频率为22%。生物活性相关性分析结果表明,供试稗草种群对氰氟草酯与唑酰草胺、敌稗、二氯喹啉酸存在极强相关性,与五氟磺草胺存在强相关性,与嘧啶肟草醚存在弱相关性。[结论]研究发现黑龙江地区稻田稗草对氰氟草酯已产生普遍抗性,且对多种常用除草剂存在交互和多抗性,嘧啶肟草醚可以作为合适的替代轮换药剂。[Aims]These experiments were designed to study the resistance level of Echinochloa crus-galli to cyhalofop-butyl in Heilongjiang Province,and identify the potential rotational herbicides.[Methods]The single-dose method and whole-plant bioassay were used to detected the resistance levels of 33 populations of E.crus-galli,and the cross and multiple resistance to other frequently used herbicides.[Results]The results of single-dose method showed that except HL33 from wasteland,relative fresh weigh of the other 32 populations were greater than 25%,which could be inferred as suspected resistant populations.The results of resistance showed that 3 populations showed high level of resistance with RI>10,accounting for 33%;4 populations with RI ranged from 5.28 to 9.96,showed medium level of resistance,accounting for 44%;2 populations showed low level resistance with RI<5,and the frequency was 22%.The results of correlation analysis of biological activity showed that cyhalofop-butyl had an extremely strong correlation with metamifop,propanil and quinclorac,it had a stong correlation with penoxsulam,while it had a weak correlation with pyribenzoxim.[Conclusions]E.crus-galli in Heilongjiang Province has developed a general resistance to cyhalofop-butyl,which showed cross and multiple resistance to several frequentlyused herbicides in rice the fields.Pyribenzoxim can be used as a suitable rotation agent.

关 键 词：稻田稗草 氰氟草酯 抗性水平 药剂筛选 

分 类 号：S482.4[农业科学—农药学]