干旱胁迫对黑骨藤种子萌发及生理特性的影响  

Effects of Drought Stress on Seed Germination and Physiological Characteristics of Periploca forrestii Schltr Seed

作　　者：何艳妮 梁明芝 龙金兰 吴之坤 HE Yanli;LIANG Mingzhi;LONG Jinlan;WU Zhikun(Guizhou University of Traditional Chinese Medicine,Guiyang 550025)

机构地区：[1]贵州中医药大学,贵阳550025

出　　处：《耕作与栽培》2024年第3期5-9,18,共6页Tillage and Cultivation

基　　金：国家自然科学基金委员会-贵州省人民政府联合基金项目(U1812403-2);贵州省科技计划项目(黔科中引地[2022]4016)。

摘　　要：为探究干旱胁迫对黑骨藤种子萌发的影响,本研究采用不同浓度梯度的PEG-6000溶液对黑骨藤种子进行胁迫实验,分析不同浓度PEG-6000胁迫环境下的种子萌发相关指标,比较不同浓度PEG-6000胁迫下种子萌发特性的差异。结果表明,在中低浓度胁迫下,黑骨藤种子有一定的抗逆性,而高浓度则抑制种子萌发,其萌发率、发芽势、发芽指数、逐日萌发率等随PEG-6000浓度的增加而下降,高度干旱胁迫对种子萌发有抑制作用;随着浓度的增大,黑骨藤幼苗的根长、茎长和苗高均受到抑制而逐渐降低。丙二醛含量与PEG-6000浓度成正比,总体呈现上升趋势;中低浓度的PEG-6000处理的种子蛋白质含量随PEG-6000浓度增加而逐渐上升,高浓度处理则下降;可溶性糖含量随PEG-6000浓度变化基本趋于一致。综合分析表明,重度干旱会抑制黑骨藤种子萌发,在今后的黑骨藤引种驯化过程中应适度控制PEG-6000浓度。In order to explore the effect of drought stress on seed germination of Periploca forrestii Schltr,PEG-6000 solution with different concentration gradients was used to conduct stress experiments on Periploca forrestii seeds,and the relevant indexes of seed germination under different concentrations of PEG-6000 stress were analyzed,the differences in seed germination characteristics under different concentrations of PEG-6000 stress were compared.The results showed that under medium and low concentration drought stress,the seeds of Periploca forrestii had a certain degree of stress resistance,while the high concentration of PEG-6000 inhibited seed germination,its germination rate,germination potential,germination index and daily germination rate decreased with the increase of PEG-6000 concentration,and high drought stress had an inhibitory effect on seed germination.With the increase of PEG-6000 concentration,the root length,stem length and seedling height of the seeds germinated by the seeds of Periploca forrestii were inhibited and gradually decreased.The malondialdehyde content was proportional to the concentration of PEG-6000,and the overall trend was upward.The protein of seeds treated with low and medium concentrations of PEG-6000 gradually increased with the increase of concentration,while that of high concentration treatment decreased.The soluble saccharide content tended to be consistent with the concentration of PEG-6000.The comprehensive analysis showed that severe drought would inhibit the seed germination of Periploca forrestii,so the concentration of PEG-6000 should be moderately controlled in the future introduction and domestication process of Periploca forrestii.

关 键 词：黑骨藤 干旱胁迫 萌发指标 生理指标 

分 类 号：S567.239[农业科学—中草药栽培]