钙离子胁迫对天门冬种子萌发生理特性的影响  

Effects of Calcium Stress on Physiological Characteristics of Asparagus cochinchinensis Seed Germination

作　　者：陈恋 雷阳 韩雪 刘海[3] 檀龙颜 CHEN Lian;LEI Yang;HAN Xue;LIU Hai;TAN Longyan(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;Guizhou Vocational College of Agriculture,Guiyang 551400,China;Guizhou Academy of Agricultural Sciences,Guiyang 550006,China)

机构地区：[1]贵州中医药大学,贵阳550025 [2]贵州农业职业学院,贵阳551400 [3]贵州省农业科学院,贵阳550006

出　　处：《耕作与栽培》2024年第3期60-65,共6页Tillage and Cultivation

基　　金：贵州中医药大学大学生创新创业训练计划项目(贵中医大创合字[2023]35号);国家级大创项目(202310662136)。

摘　　要：为研究天门冬种子萌发响应钙离子胁迫的生理机制,以天门冬种子为试验材料。以滤纸为发芽床,在直径20 cm的培养皿中培养天门冬种子,置人工气候室中培养,培养温度为25℃,黑暗培养。浇施含不同浓度(0、75、150 mmol/L)钙离子的霍格兰德营养液,观察萌发种子生理特性。结果表明,随着处理浓度的增加,天门冬萌发种子的含水量呈下降趋势;电导率、总可溶性糖、脯氨酸、丙二醛、过氧化氢、超氧阴离子含量、抗氧化酶活性均呈上升趋势。以上研究结果表明,钙离子胁迫可能对天门冬萌发种子细胞产生渗透胁迫,进一步导致氧化胁迫的产生;天门冬萌发种子可能通过合成渗透保护物质调节细胞的渗透压,并通过抗氧化酶清除活性氧。In order to study the physiological mechanism of Asparagus cochinchinensis seed germination in response to calcium ion stress,the seeds were cultured in a culture dish with a diameter of 20 cm on filter paper and cultured in an artificial climate chamber at 25℃in the dark.Hoagland nutrient solution containing different concentration of calcium ions(0,75,150 mmol/L)was poured,and the physiological characteristics of germinated seeds were observed.The results showed that with the increase of treatment concentration,the water content of the germinated seeds decreased.And the electronic conductivity,the content of total soluble sugar,proline,malondialdehyde,hydrogen peroxide,superoxide anion,the activity of antioxidant enzymes all showed an increasing trend.The results indicated that calcium ion stress may induce osmotic stress to the germination seed cells and further lead to oxidative stress.The germinated seeds may regulate the osmotic pressure of cells by synthesizing osmoprotective substance and scavenging reactive oxygen species by antioxidant enzymes.

关 键 词：天门冬 种子萌发 钙离子 渗透胁迫 氧化性损伤 

分 类 号：Q945.78[生物学—植物学]