槲寄生UPLC指纹图谱研究  

Study on Ultra High Performance Liquid Chromatography Fingerprint of Mistletoe

作　　者：张奡锴 李文杰[1] 董扬扬 褚寒冰 董学海 赵丽艳 张万明[1] 张元元 ZHANG Ao-kai;LI Wen-jie;DONG Yang-yang;CHU Han-bing;DONG Xue-hai;ZHAO Li-yan;ZHANG Wan-ming;ZHANG Yuan-yuan(Department of Pharmacy,Hebei North University,Hebei Key Laboratory of Neuropharmacology,Zhangjiakou,075000,China)

机构地区：[1]河北北方学院药学院,河北省神经药理学重点实验室,张家口075000

出　　处：《神经药理学报》2024年第3期1-8,共8页Acta Neuropharmacologica

基　　金：河北省大学生创新创业训练计划项目(No.202310092010);河北省自然科学基金项目(No.H2023405003);河北省教育厅高校基本科研业务费(No.JYT2020002);河北省卫生健康委科研基金项目(No.20180812)。

摘　　要：目的:建立槲寄生超高效液相色谱(ultra performance liquid chromatography,UPLC)指纹图谱并进行相似度评价,结合聚类分析(cluster analysis,CA)和主成分分析(principal component analysis,PCA)化学计量学分析方法,为槲寄生药材质量一致性评价提供参考。方法:色谱柱为Waters ACQUITY UPLC HSS T3(2.1×100 mm,1.8μm);流动相为乙腈(A)-0.5%乙酸水(B),梯度洗脱;检测波长254 nm;柱温30℃;进样量3μL。采用《中药色谱指纹图谱相似度评价系统软件(2012版)》建立槲寄生指纹图谱,标定其共有峰,并进行相似度评价;采用SPSS 26.0对14批不同产地的槲寄生进行聚类分析和主成分分析。结果:槲寄生指纹图谱的方法学考察结果良好,确定了14个共有峰,通过与对照品比对,指认了其中4号峰为紫丁香苷、9号峰为高圣草素-7-O-β-D-葡萄糖苷。相似度评价结果显示,11批样品的相似度在0.950以上,其余3批相似度小于0.950;通过聚类分析将样品聚为3类,S5单独为一类,S12,S7,S9为一类,其余样品聚为一类;主成分分析得到的4个主成分因子的累计方差贡献率为88.732%,其中S5、S10、S3、S11样品的主成分因子在综合得分位于前四位,整体质量良好。结论:该研究所建立的指纹图谱方法分离度、精密度、稳定性良好,分析时间短,并且结合相似度评价、聚类分析和主成分分析,为槲寄生药材的质量控制提供理论基础。Objective:The UPLC fingerprint of Mistletoe was established and the similarity evaluation was carried out.Combined with cluster analysis and principal component analysis,chemometric analysis methods were used to provide reference for the quality consistency evaluation of Mistletoe medicinal materials.Methods:The chromatographic column was Waters ACQUITY UPLC HSS T3 chromatographic column(2.1×100 mm,1.8μm).The mobile phase was acetonitrile(A)-0.5%acetic acid water(B)with gradient elution.The detection wavelength was 254 nm.Column temperature 30℃;the injection volume was 3μL.《The Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 Edition)》was used to establish the fingerprint of Mistletoe,and the common peaks were calibrated and the similarity was evaluated.Cluster analysis and principal component analysis were performed on 14 batches of Mistletoe by SPSS 26.0.Results:The methodological investigation results of the fingerprint of Mistletoe were good,and 14 common peaks were identified.By comparing with the reference substance,it was identified that the No.4 peak was Syringin and the No.9 peak was homoeriodictyol-7-O-β-D-glucoside.The similarity evaluation results showed that the similarity of 11 batches of samples was above 0.950,and the similarity of the other 3 batches was less than 0.950.The samples were clustered into three categories by cluster analysis,S5 was a separate category,S12,S7,S9 were a category,and the rest of the samples were clustered into one category.The cumulative variance contribution rate of the four principal component factors obtained by principal component analysis was 88.732%.The principal component factors of S5,S10,S3 and S11 samples were in the top four of the comprehensive scores,and the overall quality was good.Conclusion:The fingerprint method established in this study has good separation,precision and stability,short analysis time and strong specificity.Combined with similarity evaluation,cluster analysis and principal compon

关 键 词：槲寄生 UPLC 指纹图谱 聚类分析 主成分分析 相似度评价 质量控制 

分 类 号：R917[医药卫生—药物分析学]