孕期低剂量镉暴露对C57BL/6母鼠肾脏组织的毒性损伤作用  

Toxic Effects of Gestational Cadmium Exposure on the Maternal Kidneys

作　　者：刘建军 许鹏[2,3,4] Liu Jianjun;Xu Peng(Lyuliang Comprehensive Test Center,Lvliang 033000,Shanxi,China;School of Life Science,Shanxi University,Taiyuan 030006,Shanxi,China;Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Shanxi University,Taiyuan 030006,Shanxi,China;Lyuliang People's Hospital,Lyuliang 033099,Shanxi,China)

机构地区：[1]吕梁市综合检验检测中心,山西吕梁033000 [2]山西大学生命科学学院,山西太原030006 [3]山西大学化学生物学与分子工程教育部重点实验室,山西太原030006 [4]山西省吕梁市人民医院,山西吕梁033099

出　　处：《农业技术与装备》2024年第7期109-111,114,共4页Agricultural Technology & Equipment

基　　金：国家自然科学基金(No.32371715);中央引导地方科技发展资金(No.YDZJSX2022A002);吕梁市引进高层次科技人才重点研发项目(2023RC07)。

摘　　要：为研究孕期低剂量镉暴露对母鼠肾脏的毒性效应,基于前期构建的孕期低剂量镉暴露小鼠模型,通过组织显微切片技术对镉暴露母鼠的肾脏组织结构进行观察,并通过实时定量PCR法和蛋白质免疫印迹法对肾脏中炎症相关基因和凋亡调控基因的mRNA和蛋白质表达水平进行检测。实验结果显示:孕期低剂量镉暴露可导致肾脏结构出现一定程度的病变,如肾小球内细胞数目增多,肾小囊变窄,肾小球略微肿胀等;与对照组相比,镉暴露母鼠肾脏中凋亡功能调控基因Bax的mRNA和蛋白质表达水平显著升高。综上所述,孕期低剂量镉暴露可导致母体肾脏结构发生损伤,该毒性作用可能与凋亡调控基因Bax的表达水平升高相关。研究结果旨在阐明孕期低剂量镉暴露对母体器官的毒性效应机制,为镉暴露下家畜的母胎健康风险评估和环境管理提供基础数据支持。This study explored the toxic effects of low-dose maternal Cadmium(Cd)exposure on the kidneys of mice.The histological observation of kidneys of the Cd-exposed mice was performed by hematoxylin and eosin staining method.The mRNA levels and protein levels of inflammatory factors and apoptotic genes were detected by the quantitative real-time PCR and western blot methods.The results showed that low-dose maternal Cd exposure can lead to renal structural changes,such as the increased number of the glomerulus cells,the slight swelling of the glomerulus,the mRNA and protein levels of Bax were significantly increased in the maternal kidneys of Cd-exposed groups.In summary,low-dose Cd exposure can lead to the structural damages of maternal kidneys,which is likely to be due to the increase in the expression levels of Bax.This study may provide basic data for disease research in animal husbandry.

关 键 词：孕期暴露 肾脏 C57BL/6小鼠 镉 

分 类 号：S852.3[农业科学—基础兽医学]