定喘汤对痰热阻肺型慢阻肺大鼠NF-κB、TNF-α和MCP-1水平的影响  

Effect of Dingchuan Decoction on NF-κB,TNF-αand MCP-1 in Rats with Chronic Obstructive Pulmonary Disease of Phlegm Heat Obstructing Lung Syndrome

作　　者：李重 胡李慧 周建军 程俊敏 LI Zhong;HU Lihui;ZHOU Jianjun;CHENG Junmin(Department of Geriatric Medicine,Jiangxi Provincial Hospital of Integrated Traditional Chinese and Western Medicine/The Fourth Affiliated Hospital of Jiangxi University of Chinese Medicine,Jiangxi Province,Nanchang 330000,China;Department of Respiratory Medicine,Jiangxi Provincial Hospital of Integrated Traditional Chinese and Western Medicine/The Fourth Affiliated Hospital of Jiangxi University of Chinese Medicine,Jiangxi Province,Nanchang 330000,China;Department of Pulmonary Disease,Hubei Provincial Hospital of Traditional Chinese Medicine,Hubei Province,Wuhan 430074,China)

机构地区：[1]江西省中西医结合医院/江西中医药大学第四附属医院老年医学科,江西南昌330000 [2]江西省中西医结合医院/江西中医药大学第四附属医院呼吸内科,江西南昌330000 [3]湖北省中医院肺病科,湖北武汉430074

出　　处：《中国中医药现代远程教育》2024年第18期183-185,192,共4页Chinese Medicine Modern Distance Education of China

基　　金：江西省卫生健康委科技计划项目【No.202211486】。

摘　　要：目的探讨定喘汤治疗痰热阻肺型慢性阻塞性肺疾病(COPD)大鼠效果及机制。方法按随机数字表法,将30只Wistar大鼠分为对照组、模型组和定喘汤组,对照组给予正常饮食,模型组和定喘汤组给予烟熏和高脂饮食3个月。造模完成后连续给药21 d,取材。观察大鼠一般状况,苏木精-伊红(HE)染色观察肺组织病理改变,酶联免疫吸附(ELISA)法检测血清核因子κB(NF-κB)、肿瘤坏死因子-α(TNF-α)和单核细胞趋化蛋白-1(MCP-1)含量,免疫组化化学(IHC)法检测肺组织巨噬细胞聚集数量。结果与对照组比较,模型组血清NF-κB、TNF-α和MCP-1含量升高,肺组织巨噬细胞聚集增多;与模型组比较,定喘汤组上述血清指标均含量降低,肺组织巨噬细胞聚集减少。结论定喘汤可能通过减少COPD大鼠血清NF-κB、TNF-α和MCP-1含量,抑制巨噬细胞向肺组织聚集,减轻其气道炎症反应。Objective To explore the effect and mechanism of Dingchuan decoction in treating chronic obstructive pulmonary disease(COPD)rats of phlegm heat obstructing lung syndrome.Methods According to the random number table method,30 Wistar rats were divided into normal group,model group,and Dingchuan decoction group.The control group was given a normal diet,the model group and the Dingchuan decoction group were given high-fat diet with inhaling smoke leads to chronic obstructive pulmonary disease for 3 months.After the completion of modeling,the mice in each group were administrated with corresponding drugs by gavage for 3 weeks.At the end of administration,the samples were collected for the determination of various indexes,and the general condition of rats were observed as well as the pathological changes in lung tissue using hematoxylin eosin(HE)staining.Enzyme linked immunosorbent assay(ELISA)was used for detecting the content of NF-κB,TNF-αand MCP-1 in serum.Immunohistochemical(IHC)method was used to detect the number of macrophages in lung tissue.Results Compared with normal group,the contents of serum NF-κB,TNF-αand MCP-1 in model group were increased,and the aggregation of lung macrophages was increased.Compared with the model group,the above serum indexes and macrophage aggregation in lung tissue decreased in Dingchuan decoction group.Conclusion Dingchuan decoction may reduce the content of NF-κB,TNF-αand MCP-1 in serum of COPD rats,inhibit the aggregation of macrophages to lung tissue,and alleviate airway inflammation.

关 键 词：肺胀 慢性阻塞性肺疾病 痰热蕴肺证 定喘汤 中医药疗法 

分 类 号：R285.5[医药卫生—中药学]