冰薰2号薰衣草ISSR-PCR反应体系优化及遗传特征分析  

Optimization of ISSR-PCR reaction system and genetic characteristics analysis of Ice Lavandula 2 lavender

作　　者：李瑞航 李婧 马天意 郭佳欣 张梅娟[1] 彭疑芳 王韬 闫爽 赵井泉 沙伟[1] LI Rui-hang;LI Jing;MA Tian-yi;GUO Jia-xin;ZHANG Mei-juan;PENG Yi-fang;WANG Tao;YAN Shuang;ZHAO Jing-quan;SHA Wei(College of Life Science,Agriculture and Forestry,Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas,Qiqihar University,Heilongjiang Qiqihar 161006,China)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,抗性基因工程与寒地生物多样性保护黑龙江重点实验室,黑龙江齐齐哈尔161006

出　　处：《齐齐哈尔大学学报（自然科学版）》2024年第3期63-68,88,共7页Journal of Qiqihar University(Natural Science Edition)

基　　金：黑龙江省省属高等学校基本科研业务费科研项目(145109135)。

摘　　要：为探究冰薰2号薰衣草的遗传特征,采用ISSR分子标记技术在优化反应体系的基础上对冰薰2号薰衣草亲代及F1代个体进行遗传多样性分析。结果显示,优化的最佳ISSR-PCR扩增体系为:在20μL体系中,dNTP(每种2.5 mmol/L)用量为1.4μL,引物(10μmol/L)用量为0.7μL,DNA模板量为20 ng,Taq DNA聚合酶用量0.75μmol/min。选取8个ISSR引物对冰薰2号薰衣草亲代及F1代个体进行试验,根据扩增结果进行计算,并分析亲代及F1代个体间遗传多样性指数。结果表明,冰薰2号薰衣草遗传多样性水平低,遗传变异度低。For exploring the genetic characteristics of Ice Lavandula 2 lavender(Lavandula angustifolia),ISSR molecular marker technology was used for the analysis,the optimal ISSR-PCR amplification system optimized for Ice Lavandula 2 was as follows:in the 20μL system,the amount of dNTP(2.5 mmol/L each)was 1.4μL,the amount of primer(10μmol/L)was 0.7μL,the amount of DNA template was 20 ng,and the amount of Taq DNA polymerase was 0.75μmol/min.Eight ISSR primers were selected for the tests of the parents and F1 generation individuals of Ice Lavandula 2,and the genetic diversity index of the parents and F1 generation individuals was calculated and analyzed according to the amplified results.The results indicated that the genetic diversity and genetic variation of Ice Lavandula 2 was low.

关 键 词：薰衣草 ISSR 分子标记 遗传特征 遗传多样性 

分 类 号：Q37[生物学—遗传学]