8个大豆SERK基因干旱胁迫表达分析  

Expression analysis of 8 SERK genes in soybean under drought stress

作　　者：关鑫宇 林阳 陈炯辛 马洪宇 张勇[2] 翟莹[1] GUAN Xin-yu;LIN Yang;CHEN Jiong-xin;MA Hong-yu;ZHANG Yong;ZHAI Ying(College of Life Science and Agroforestry,Qiqihar University,Heilongjiang Qiqihar 161006,China;Keshan Branch of Heilongjiang Academy of Agricultural Sciences,Heilongjiang Qiqihar 161606,China)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006 [2]黑龙江省农业科学院克山分院,黑龙江齐齐哈尔161606

出　　处：《齐齐哈尔大学学报（自然科学版）》2024年第4期75-80,共6页Journal of Qiqihar University(Natural Science Edition)

基　　金：齐齐哈尔大学大学生创新创业训练计划项目(x202310232033);黑龙江省省属高等学校基本科研业务费科研项目(145109506)。

摘　　要：对大豆中的8个GmSERK基因及其启动子序列进行生物信息学分析,通过实时荧光定量RT-PCR(qRT-PCR)对基因在干旱胁迫下的表达量进行检测。结果显示,8个GmSERK基因分别来自大豆基因组的5条染色体,它们编码的蛋白含有596~624个氨基酸。8个GmSERK蛋白均含有1个信号肽序列和多个亮氨酸富集重复序列(LRR)。除GmSERK10蛋白不具有跨膜结构域外,其他GmSERK蛋白均含有1个跨膜结构域和1个激酶结构域。除GmSERK7外,其余7个GmSERK基因在干旱胁迫下的表达量均发生变化,表明大豆SERK基因家族中的部分基因能够响应干旱胁迫。8个GmSERK启动子中共含有5种激素响应相关元件(ABRE、CGTCA-motif、P-box、TATC-box和TCA-element)和3种胁迫响应相关元件(ARE、MBS和TC-rich repeats),但不同启动子中含有的元件种类和数量各不相同。In this study,eight soybean GmSERK genes and their promoter sequences were analyzed by bioinformatics,and their expression levels under drought stress were detected by real-time fluorescence quantitative RT-PCR(qRT-PCR).The results showed that eight GmSERK genes were located on five chromosomes of soybean genome,respectively,and the proteins they encoded containing 596-624 amino acids.The eight GmSERK proteins all contained one signal peptide sequence and multiple leucine-enriched repeats(LRR).Except for GmSERK10 protein,all other GmSERK proteins contain one transmembrane domain and one kinase domain.Except for GmSERK7,the expression levels of the other 7 GmSERK genes changed under drought stress,indicating that some soybean GmSERK genes could respond to drought stress.The eight GmSERK promoters contained five type of hormone response-related elements(ABRE,CGTCA-motif,P-box,TATC-box and TCA-element)and three type of stress response-related elements(ARE,MBS and TC-rich repeats).However,the type and number of elements contained in different promoters are different.

关 键 词：大豆 SERK 生物信息学分析 干旱胁迫 启动子 

分 类 号：S565.1[农业科学—作物学]