基于ISSR标记的冰薰1号薰衣草亲本及其F1代遗传多样性分析  

Genetic characteristics of IL1 lavender based on ISSR molecular markers

作　　者：郭佳欣 李瑞航 马天意 张梅娟[1,2] 蔡璨 王韬 彭疑芳 黄荣雁 阎爽 赵井泉 沙伟 GUO Jia-xin;LI Rui-hang;MA Tian-yi;ZHANG Mei-juan;CAI Can;WANG Tao;PENG Yi-fang;HUANG Rong-yan;YAN Shuang;ZHAO Jing-quan;SHA Wei(College of Life Science,Agriculture and Forestry,Qiqihar University,Heilongjiang Qiqihar 161006,China;Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas,Heilongjiang Qiqihar 161006,China)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006 [2]抗性基因工程与寒地生物多样性保护黑龙江重点实验室,黑龙江齐齐哈尔161006

出　　处：《齐齐哈尔大学学报（自然科学版）》2024年第1期67-74,共8页Journal of Qiqihar University(Natural Science Edition)

基　　金：黑龙江省省属高等学校基本科研业务费科研项目(145109135)。

摘　　要：为探究实验室培育的冰薰1号薰衣草的亲代和子代的遗传多样性,选用32株冰薰1号植株为亲本,及其中6株亲本的F1代群体为试验材料,在进行PCR反应体系优化的基础上,采用ISSR分子标记技术对冰薰1号的亲本群体及自由授粉的F1代群体进行遗传多样性分析。筛选的最佳PCR反应体系为:总体积20μL,rTaq 1.0μmol/min,引物浓度为0.7μmol/L,dNTP浓度为0.3 mmol/L,DNA模板浓度为40 ng/μL,最适反应循环数为35。ISSR分析结果表明,冰薰1号薰衣草个体间遗传距离较近,遗传多样性水平较低,遗传稳定。For exploring the genetic diversity of parents and offspring of IL1 lavender cultivated from our laboratory,32 IL1 plants were selected as parents,and the F1 populations of 6 parents were used as experimental materials.Based on the optimization of PCR reaction system,ISSR molecular marker technology was used to analyze the genetic diversity of the parent population and the F1 population of free pollination of IL1 plants.The optimal system was as follows:total volume of 20μL,rTaq of 1.0μmol/min,primer concentration of 0.7μmol/L,dNTP concentration of 0.3 mmol/L,DNA template concentration of 40 ng/μL,and the optimal reaction cycle number was 35.The ISSR analysis results showed the genetic distance between lavender individuals of IL1 lavender was close,the level of genetic diversity was low,and the genetic stability was stable.

关 键 词：薰衣草 ISSR-PCR 体系优化 遗传特征 

分 类 号：Q37[生物学—遗传学]