灰色关联度分析清半夏体外抗氧化作用的谱效关系  

Grey correlation analysis of the spectrum effect relationship of in vitro antioxidant effec of Pinellia Rhizoma praeparatum cum alumine

作　　者：张国玉 熊继东 魏家艳 仲楠 强宇靖 李芸 ZHANG Guoyu;XIONG Jidong;WEI Jiayan;ZHONG Nan;QIANG Yujing;LI Yun(Gansu Pharmaceutical Industry Innovation Research Institute,Northwest Collaborative Innovation Center for Traditional Chinese Medicine,College of Pharmacy,Gansu University of Chinese Medicine,Lanzhou,Gansu,730000 P.R.China)

机构地区：[1]甘肃中医药大学药学院,西北中藏药省部共建协同创新中心,陇药产业创新研究院,甘肃兰州730000

出　　处：《华西药学杂志》2024年第4期382-386,共5页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:82360773);中药材及饮片质量控制重点实验室项目(2021GSMPA-KL08);技术创新引导计划—农业类项目(22CX8NK244);甘肃省中药炮制技术传承基地建设项目(2022)。

摘　　要：目的通过灰色关联度分析12批清半夏与其体外抗氧化作用间的谱效关系,寻找其体外抗氧化作用的物质基础。方法建立清半夏的HPLC指纹图谱,以DPPH、ABTS自由基清除率及总还原能力的半抑制浓度为药效指标,采用灰色关联度分析其谱效关系。结果建立的指纹图谱中确定了7个共有峰,并指认了6个,相似度均>0.977。聚类分析将12批清半夏饮片分为5类。对12批清半夏与体外抗氧化作用进行谱效关联,其关联度均>0.6,表明其体外抗氧化作用是由多种成分共同作用,对其贡献较大的为峰1(草酸)、峰6(鸟苷)、峰3(腺嘌呤)。结论清半夏体外抗氧化作用的主要成分为草酸、鸟苷、腺嘌呤,为清半夏抗氧化作用机制的深入研究提供了依据。OBJECTIVE To analyze the crrelation between 12 batches of Pinellia Rhizoma praeparatum cum alumine(PRPCA)and its antioxidant activity in vitro by grey correlation to find the material basis of its antioxidant activity.METHODS The HPLC fingerprint of PRPCA was established,and the ICso values of DPPH,ABTS free radical scavenging rate and total reducing power were used as pharmacodynamic indexes.RESULTS The established fingerprint identified 7 common peaks and 6 peaks,and the similarity was greater than 0.977.The 12 batches of PRPCA decoction were divided into 5 categories by cluster analysis.The spectral correlation between 12 batches of PRPCA and the antioxidant effect in vitro was greater than O.6,indicating that the antioxidant effect in vitro was caused by the combined action of various components,and the major contributions were peak 1(oxalic acid),peak 6(guanosine)and peak 3(adenine).CONCLUSION The main components of antioxidation in vitro may be oxalic acid,guanosine and adenine,which provides scientific basis for further study of antioxidation mechanism of PRPCA.

关 键 词：清半夏 半夏 指纹图谱 抗氧化活性 谱效关联 灰色关联度 草酸 鸟苷 腺嘌呤 

分 类 号：R96[医药卫生—药理学]