巴西橡胶树HbCPT7基因的克隆和亚细胞定位分析  

Cloning and Subcellular Localization of HbCPT7 Gene from Hevea brasiliensis

作　　者：王澳盛 刘晓东 朱芳玉 王艺玮 唐朝荣 曹杰 Wang Aosheng;Liu Xiaodong;Zhu Fangyu;Wang Yiwei;Tang Chaorong;Cao Jie(Natural Rubber Provincial and Ministerial Collaborative Innovation Center,College of Tropical Crops,Hainan University,Haikou,570228)

机构地区：[1]海南大学热带作物学院,天然橡胶省部共建协同创新中心,海口570228

出　　处：《分子植物育种》2024年第15期4957-4964,共8页Molecular Plant Breeding

基　　金：国家自然科学基金青年基金项目(32101543)资助。

摘　　要：顺式异戊烯基转移酶(cis-prenyltransferase, CPT)是催化天然橡胶生物合成的关键酶之一,本研究克隆了橡胶树(Hevea brasiliensis)胶乳中高表达的顺式异戊烯基转移酶基因HbCPT7,并进行了组织表达模式、保守结构域和亚细胞定位研究。qPCR分析表明HbCPT7在胶乳中特异表达,蛋白质保守结构域分析发现Hb CPT7具有CPTs基因家族的5个高度保守的结构域,表明其为一个结构完整的CPT酶。亚细胞定位分析发现HbCPT7定位在细胞核和细胞质中,且细胞质中的部分定位信号与内质网重合,推测HbCPT7定位在内质网,进而参与天然橡胶的生物合成。以上研究结果为进一步阐明HbCPT7的功能提供线索。Wang Aosheng;Liu Xiaodong;Zhu Fangyu;Wang Yiwei;Tang Chaorong;Cao Jie;Natural Rubber Provincial and Ministerial Collaborative Innovation Center,College of Tropical Crops,Hainan University;Cis-prenyltransferase(CPT) is one of the key enzymes that catalyzes the biosynthesis of natural rubber,in the latex cells of Hevea brasiliensis, here, we cloned HbCPT7, and analyzed the tissue expression patterns,conserved domains and subcellular localization of HbCPT7. The results of qPCR analysis showed that HbCPT7was specifically expressed in latex. The analysis of protein conserved domains found that HbCPT7 has five highly conserved domains of the CPTs family, indicating that is a CPT enzyme with complete structure. Subcellular localization analysis showed that HbCPT7 was located in the nucleus and cytoplasm, and part of the localization signal in the cytoplasm coincided with the endoplasmic reticulum, inferred HbCPT7 was located in the endoplasmic reticulum, and participated in natural rubber biosynthesis of Hevea brasiliensis. Above results provided a clue for further exploring the function of HbCPT7.

关 键 词：天然橡胶 巴西橡胶树 顺式异戊烯基转移酶基因HbCPT7 亚细胞定位 

分 类 号：S794.1[农业科学—林木遗传育种]